Alkaline Phosphatase Activity in Cholesteatoma Epithelium and Skin
| Autor: | K Dammert, A Palva, Tauno Palva |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
medicine.medical_specialty
food.ingredient Iodoacetic acid Iodoacetates In Vitro Techniques Skin Diseases Lecithin Epithelium chemistry.chemical_compound food Internal medicine Keratin otorhinolaryngologic diseases medicine Humans Cysteine Sulfhydryl Compounds Cholesteatoma Ear Diseases chemistry.chemical_classification biology Histocytochemistry Acid phosphatase Hydrogen-Ion Concentration Alkaline Phosphatase medicine.disease Molecular biology Enzyme assay medicine.anatomical_structure Endocrinology Otorhinolaryngology chemistry Glycerophosphates Phosphatidylcholines biology.protein Keratins Alkaline phosphatase |
| Zdroj: | ORL. 34:153-159 |
| ISSN: | 1423-0275 0301-1569 |
| DOI: | 10.1159/000275043 |
| Popis: | Alkaline phosphatase activity was demonstrated in the granular cell layer of cholesteatoma epithelium, ear canal and postauricularin skin by blocking the cysteine-containing active sulfhydryl groups with iodoacetic acid. Alkaline phosphatase activity occurred only at pH 9.4. Substitution of lecithin for glycerophosphate did not result in demonstration of enzyme activity. Since alkaline phosphatase activity is very strong in columnar epithelium, it is speculated that inhibition of its activity may be required for the formation of keratinizing epithelium. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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