The utility of measuring C-terminal telopeptides of collagen type II (CTX-II) in serum and synovial fluid samples for estimation of articular cartilage status in experimental models of destructive joint diseases
| Autor: | S. Oestergaard, Per Qvist, Luc Chouinard, Susan Y. Smith, László B. Tankó, Morten A. Karsdal, Nancy Doyle |
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| Rok vydání: | 2006 |
| Předmět: |
Cartilage
Articular Pathology medicine.medical_specialty Biomedical Engineering Type II collagen Arthritis Enzyme-Linked Immunosorbent Assay Osteoarthritis Collagen Type I Rheumatology In vivo Synovial Fluid medicine Animals Synovial fluid Orthopedics and Sports Medicine Collagen Type II Cartilage oligomeric matrix protein Microscopy medicine.diagnostic_test biology Chemistry Cartilage Reproducibility of Results medicine.disease Arthritis Experimental Mono-iodoacetate-induced arthritis Rats Disease Models Animal medicine.anatomical_structure Immunoassay C-telopeptide of collagen type II biology.protein Collagen-induced arthritis Joints Peptides |
| Zdroj: | Osteoarthritis and Cartilage. 14:670-679 |
| ISSN: | 1063-4584 |
| DOI: | 10.1016/j.joca.2006.01.004 |
| Popis: | SummaryObjectiveTo characterize and validate a novel, enzyme-linked immunoassay for measuring cross-linked dimer forms of C-terminal telopeptides of type II collagen (CTX-II) in serum and synovial fluid of rodents, and investigate whether CTX-II measurements can reflect joint status in two established animal models of destructive joint diseases.MethodsFirstly, the specificity, in vivo validity, antigen recovery, and reproducibility of the assay were investigated. Secondly, we induced arthritis in rats using either bovine collagen type II or mono-iodoacetate. CTX-II levels were measured in the serum and synovial fluid of the affected femoro-tibial joint and correlated with microscopic severity of joint lesions as determined by validated scoring systems.ResultsThe F4601 monoclonal antibody (mAb) is highly specific for the EKGPDP sequence at the CTX-II. Strong CTX-II signals were detected during enzymatic degradation of articular cartilage explants by matrix metalloproteinase (MMP)-9 or MMP-13. The assay presented a good degree of precision and reproducibility (inter- and intra-assay CVs |
| Databáze: | OpenAIRE |
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