Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method
| Autor: | Tetsushi Yoshikawa, Ayano Fujita, Shiho Akimoto, Masaru Ihira, Yoshizo Asano, Takao Ozaki, Ken Sugata, Fumi Miyake, Masahiro Ohashi, Sadao Suga, Naoko Nishimura |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Fever Herpesvirus 6 Human viruses Loop-mediated isothermal amplification Exanthema Subitum Biology Sensitivity and Specificity law.invention chemistry.chemical_compound law Virology Humans Dna viral Polymerase chain reaction DNA Primers Infant virus diseases Nucleic acid amplification technique biology.organism_classification DNA extraction Infectious Diseases chemistry DNA Viral Female Human herpesvirus 6 Nucleic Acid Amplification Techniques DNA |
| Zdroj: | Journal of Clinical Virology. 39:22-26 |
| ISSN: | 1386-6532 |
| DOI: | 10.1016/j.jcv.2007.02.001 |
| Popis: | Background A more rapid and easier method is needed for monitoring human herpesvirus 6 (HHV-6) infections. The loop-mediated isothermal amplification method (LAMP) can detect viral DNA with high specificity, efficiency, and speed under isothermal conditions. LAMP requires only simple equipment that is available in hospital laboratories. Objectives We evaluated LAMP as a means of detecting HHV-6 DNA directly from patients’ sera. Results The sensitivity of the HHV-6 LAMP protocol without heat denaturation was 1000 copies/tube; with heat denaturation 10 copies/tube were detected. Three hundred serum samples from children with fever were analyzed. Using HHV-6 isolation as a definition of HHV-6 infection, the sensitivity, specificity, positive predictive value, and negative predictive value of the HHV-6 LAMP method without DNA extraction were 95.5%, 95.2%, 94.0%, and 96.4%, respectively. Conclusion Direct detection of HHV-6 DNA in serum with a modified HHV-6 LAMP could be used for rapid diagnosis of exanthem subitum (ES). |
| Databáze: | OpenAIRE |
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