Development of microsatellite markers for Manilkara maxima T.D. Penn. (Sapotaceae) and their use in conservation genetics
| Autor: | Daniele de Souza França, Fernanda Amato Gaiotto, Ramiris César Souza Moraes, José Audenor Silva-Junior |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Conservation genetics Genetic Markers Conservation of Natural Resources Genotype Endangered species Population genetics Biology Genes Plant 010603 evolutionary biology 01 natural sciences Molecular ecology 03 medical and health sciences Genetics Molecular Biology Manilkara Genetic diversity Polymorphism Genetic Ecology Genetic Variation High-Throughput Nucleotide Sequencing General Medicine Sequence Analysis DNA biology.organism_classification 030104 developmental biology Manilkara maxima Microsatellite Conservation biology Microsatellite Repeats |
| Zdroj: | Molecular biology reports. 43(6) |
| ISSN: | 1573-4978 |
| Popis: | Manilkara maxima is an endemic tree species of the Atlantic Forest in southern Bahia, Brazil. It is considered important for forest conservation due to its mutualistic interactions with endemic and endangered animals. Our aim was to develop microsatellite markers to estimate genetic diversity in order to provide information for effectiveness of future conservation programs. We used next generation sequencing technology to develop the first specific microsatellite markers for M. maxima. Seventeen new microsatellite loci were applied in 72 individuals sampled in three natural populations. On average, the number of alleles per loci was 8.8. The expected heterozygosity varied between 0.72 and 0.77, indicating that the developed set of molecular markers is useful for genetic diversity studies. Additionally, the estimated value for the combined probability of exclusion (Q) was greater than 0.999, which indicates the powerful of these molecular tools for paternity and kinship analysis. Our results demonstrate that the set of microsatellites developed in this work is a powerful tool for population genetics, molecular ecology and conservation biology purposes. |
| Databáze: | OpenAIRE |
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