Study of vortex-assisted MSPD and LC-MS/MS using alternative solid supports for pharmaceutical extraction from marketed fish
| Autor: | Karina Lotz Soares, Ednei Gilberto Primel, Sergiane Souza Caldas, Gabriel Ianzer Hertzog |
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| Rok vydání: | 2015 |
| Předmět: |
Detection limit
Spectrometry Mass Electrospray Ionization Chromatography Chemistry Electrospray ionization Extraction (chemistry) Fishes Reproducibility of Results Mass spectrometry Biochemistry Analytical Chemistry Matrix (chemical analysis) Chitosan chemistry.chemical_compound Pharmaceutical Preparations Limit of Detection Tandem Mass Spectrometry Reagent Sodium sulfate Animals Chromatography Liquid |
| Zdroj: | Analytical and Bioanalytical Chemistry. 407:4793-4803 |
| ISSN: | 1618-2650 1618-2642 |
| Popis: | A procedure based on vortex-assisted matrix solid-phase dispersion (MSPD) for the extraction of 15 pharmaceuticals from fish samples with determination by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was validated. Florisil, C18, diatomaceous earth, chitin, and chitosan were evaluated as solid supports. Best results were obtained with 0.5 g of diatomaceous earth, 0.5 g of sodium sulfate, and 5 mL of methanol. Analytical recoveries ranged from 58 to 128 % with relative standard deviation (RSD) lower than 15 %. Limit of quantification (LOQ) values for the 15 compounds ranged from 5 to 1000 ng g(-1). The method under investigation has shown to be a simple and fast extraction tool with minimum instrumentation and low amount of reagent, resulting in method low cost. Besides, alternative materials, such as chitin and chitosan, which were applied to the dispersion step for the first time, were found to be interesting alternatives. |
| Databáze: | OpenAIRE |
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