Hepatoprotective evaluation and isolation of the major secondary metabolites from the ethyl acetate extract of liquid culture filtrate of Chaetomium globosum
| Autor: | Nagwa E. Awad, Hanaa A. Kassem, Mohamed A. A. Elnaggar, Manal A. Hamed, Amal M. El-Feky |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Ethyl acetate Drug Evaluation Preclinical CCL4 Acetates Chaetomium Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound Animals Rats Wistar Pharmacology 030109 nutrition & dietetics Chromatography biology Chaetomium globosum General Medicine biology.organism_classification Malondialdehyde Rats Oxidative Stress 030104 developmental biology Biochemistry chemistry biology.protein Alkaline phosphatase Liver function Chemical and Drug Induced Liver Injury |
| Zdroj: | Biomedicinepharmacotherapy = Biomedecinepharmacotherapie. 97 |
| ISSN: | 1950-6007 |
| Popis: | The aim of the present study was to evaluate the hepatoprotective activity of ethyl acetate extract of the liquid culture filtrate of Chaetomium globosum fungus (family Chaetomiaceae). Rats were intraperitoneally injected by CCl4 (0.5ml/kg) twice a week for six consecutive weeks. Treatment tacks (250mg/kg) place at the same time of CCl4 induction and with the same duration. The evaluation was done through determination of liver function indices; aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and total serum protein content. In addition, the oxidative stress markers; hepatic glutathione content (GSH), hepatic malondialdehyde (MDA), hepatic superoxide dismutase (SOD), and hepatic total protein were estimated. Moreover, the liver architectures were also examined. Isolation and identification of the main secondary metabolites were identified. Seven volatile compounds were identified from the plain chloroform fraction where, 1-Cyclopentyl-2,2-dimethyl-1-propanol (54.63%) was presented as the major compound. Eleven compounds were also identified from the fraction eluted by chloroform: methanol (85:15). 1,5,5-Trimethyl-6-methylene-1-cyclohexene (25.79%) and Norbornan-2-one (26.84%) are presented as the major compounds of this fraction. In conclusion, the extract recorded hepatoprotective effect by ameliorating the biochemical parameters under investigation. The liver histopathological pictures confirmed our results. |
| Databáze: | OpenAIRE |
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