Administration of sesamol improved blood–brain barrier function in streptozotocin-induced diabetic rats

Autor: M. D. Chua, R. L. Ptachcinski, Jason D. Huber, Reyna L. VanGilder, Kimberly A. Kelly
Rok vydání: 2009
Předmět:
Blood Glucose
Male
medicine.medical_specialty
Antioxidant
endocrine system diseases
medicine.medical_treatment
Blood–brain barrier
medicine.disease_cause
Antioxidants
Streptozocin
Diabetes Mellitus
Experimental
Capillary Permeability
Rats
Sprague-Dawley
Lipid peroxidation
chemistry.chemical_compound
Phenols
Ethidium
Peroxynitrous Acid
Internal medicine
Diabetes mellitus
medicine
Animals
Benzodioxoles
Claudin-5
RNA
Messenger
Sesamol
Analysis of Variance
Microscopy
Confocal
General Neuroscience
Brain
Membrane Proteins
nutritional and metabolic diseases
Catalase
Phosphoproteins
Streptozotocin
medicine.disease
Rats
Disease Models
Animal
Oxidative Stress
Endocrinology
medicine.anatomical_structure
Gene Expression Regulation
chemistry
Blood-Brain Barrier
Spectrophotometry
Zonula Occludens-1 Protein
Peroxynitrite
Oxidative stress
medicine.drug
Zdroj: Experimental Brain Research. 197:23-34
ISSN: 1432-1106
0014-4819
DOI: 10.1007/s00221-009-1866-6
Popis: Uncontrolled or poorly controlled blood glucose during diabetes is an important factor in worsened vascular function. While evidence suggests that hyperglycemia-induced oxidative stress plays a prominent role in development of microangiopathy of the retina, kidney, and nerves, the role oxidative stress plays on blood-brain barrier (BBB) function and structure has lagged behind. In this study, a natural antioxidant, sesamol, was administered to streptozotocin (STZ)-induced diabetic rats to examine the role that oxidative stress plays on BBB structure and function. Experiments were conducted at 56 days after STZ injection. Male Sprague-Dawley rats randomly were divided into four treatment groups CON--control; STZ--STZ-induced diabetes; CON + S--control + sesamol; STZ + S--STZ-induced diabetes + sesamol. Functional and structural changes to the BBB were measured by in situ brain perfusion and western blot analysis of changes in tight junction protein expression. Oxidative stress markers were visualized by fluorescent confocal microscopy and assayed by spectrophotometric analysis. Results demonstrated that the increased BBB permeability observed in STZ-induced diabetic rats was attenuated in STZ + S rats to levels observed in CON. Sesamol treatment reduced the negative impact of STZ-induced diabetes on tight junction protein expression in isolated cerebral microvessels. Oxidative stress markers were elevated in STZ as compared to CON. STZ + S displayed an improved antioxidant capacity which led to a reduced expression of superoxide and peroxynitrite and reduced lipid peroxidation. In conclusion, this study showed that sesamol treatment enhanced antioxidant capacity of the diabetic brain and led to decreased perturbation of hyperglycemia-induced changes in BBB structure and function.
Databáze: OpenAIRE
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