Elasticity of Living Cells on a Microarray during the Early Stages of Adhesion Measured by Atomic Force Microscopy
| Autor: | Takaharu Okajima, Hiroshi Tokumoto, Shinichiro Hiratsuka, Yusuke Mizutani, Koichi Kawahara, Masahiro Tsuchiya |
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| Rok vydání: | 2008 |
| Předmět: |
Physics and Astronomy (miscellaneous)
Chemistry Atomic force microscope Log-normal distribution General Engineering Analytical chemistry Young's modulus General Physics and Astronomy Modulus Microarray symbols.namesake medicine.anatomical_structure Distribution function symbols Biophysics medicine Fibroblast cells Elasticity (economics) Cytoskeleton Fibroblast Elastic modulus |
| Zdroj: | Japanese Journal of Applied Physics. 47:6177-6180 |
| ISSN: | 1347-4065 0021-4922 |
| Popis: | The number distribution of the elastic modulus of fibroblast cells was successfully measured during the early stages of adhesion using an atomic force microscope (AFM) combined with a microarray as a substrate, which allowed us to arrange and culture cells so that a large number of cells could be measured in a short time period. We confirmed that the cells deposited in the wells of the microarray could be cultured for at least 12 h without any significant migration. Histograms of the Young's modulus, E, of the cells during the early stages of adhesion produced from force curve measurements of cells (n\cong300) cultured for 3–9 h were well fitted to a log-normal distribution function. With increasing incubation time, the average value of E increased significantly, while the standard deviation of the distribution remained almost constant. The results are discussed in terms of the cytoskeleton inside cells. |
| Databáze: | OpenAIRE |
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