Ex vivo construction of human primary 3D–networked osteocytes
| Autor: | Yair D Kissin, Ciaran Mannion, Qiaoling Sun, Saba Choudhary, Woo Y. Lee, Jenny Zilberberg |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Genetic Markers
Male 0301 basic medicine Histology Physiology Endocrinology Diabetes and Metabolism Cell Culture Techniques Cell Count Cell Separation Bone tissue Osteocytes Article Bone and Bones Extracellular matrix Mice 03 medical and health sciences chemistry.chemical_compound Imaging Three-Dimensional Perfusion Culture medicine Animals Humans Adaptor Proteins Signal Transducing Aged Cell Proliferation Cell growth Chemistry Cell Differentiation Anatomy Middle Aged Phenotype In vitro Cell biology Fibroblast Growth Factor-23 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Bone Morphogenetic Proteins Sclerostin Female Ex vivo |
| Zdroj: | Bone. 105:245-252 |
| ISSN: | 8756-3282 |
| DOI: | 10.1016/j.bone.2017.09.012 |
| Popis: | A human bone tissue model was developed by constructing ex vivo the 3D network of osteocytes via the biomimetic assembly of primary human osteoblastic cells with 20–25 µm microbeads and subsequent microfluidic perfusion culture. The biomimetic assembly: (1) enabled 3D-constructed cells to form cellular network via processes with an average cell-to-cell distance of 20–25 µm, and (2) inhibited cell proliferation within the interstitial confine between the microbeads while the confined cells produced extracellular matrix (ECM) to form a mechanically integrated structure. The mature osteocytic expressions of SOST and FGF23 genes became significantly higher, especially for SOST by 250 folds during 3D culture. The results validate that the bone tissue model: (1) consists of 3D cellular network of primary human osteocytes, (2) mitigates the osteoblastic differentiation and proliferation of primary osteoblast-like cells encountered in 2D culture, and (3) therefore reproduces ex vivo the phenotype of human 3D-networked osteocytes. The 3D tissue construction approach is expected to provide a clinically relevant and high-throughput means for evaluating drugs and treatments that target bone diseases with in vitro convenience. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect