In Vivo Intracellular pH Measurements in Tobacco and Arabidopsis Reveal an Unexpected pH Gradient in the Endomembrane System
| Autor: | Elias Bassil, Alexandre Martinière, Elodie Jublanc, Carine Alcon, Eduardo Blumwald, María Reguera, Hervé Sentenac, Nadine Paris |
|---|---|
| Přispěvatelé: | Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Department of Plant Sciences, University of California [Davis] (UC Davis), University of California-University of California, Dynamique Musculaire et Métabolisme (DMEM), Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM), Agropolis foundation in Montpellier, France, Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
Vacuolar Proton-Translocating ATPases Sodium-Hydrogen Exchangers Arabidopsis thaliana Endosome Intracellular pH Nicotiana tabacum Arabidopsis Plant Science Vacuole Endoplasmic Reticulum 01 natural sciences 03 medical and health sciences Tobacco [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Endomembrane system Lytic vacuole Research Articles soluble proteins 030304 developmental biology vacuolar sorting receptor 0303 health sciences biology Endoplasmic reticulum Proton-Motive Force Cell Biology Hydrogen-Ion Concentration biology.organism_classification root Cell biology Cytosol endosomal pH Vacuoles trans-Golgi 010606 plant biology & botany trans-Golgi Network |
| Zdroj: | The Plant cell The Plant cell, American Society of Plant Biologists (ASPB), 2013, 25 (10), pp.4028-43. ⟨10.1105/tpc.113.116897⟩ Plant Cell Plant Cell, American Society of Plant Biologists, 2013, 25 (10), pp.4028-43. ⟨10.1105/tpc.113.116897⟩ |
| ISSN: | 1040-4651 1532-298X |
| Popis: | The pH homeostasis of endomembranes is essential for cellular functions. In order to provide direct pH measurements in the endomembrane system lumen, we targeted genetically encoded ratiometric pH sensors to the cytosol, the endoplasmic reticulum, and the trans-Golgi, or the compartments labeled by the vacuolar sorting receptor (VSR), which includes the trans-Golgi network and prevacuoles. Using noninvasive live-cell imaging to measure pH, we show that a gradual acidification from the endoplasmic reticulum to the lytic vacuole exists, in both tobacco (Nicotiana tabacum) epidermal (ΔpH −1.5) and Arabidopsis thaliana root cells (ΔpH −2.1). The average pH in VSR compartments was intermediate between that of the trans-Golgi and the vacuole. Combining pH measurements with in vivo colocalization experiments, we found that the trans-Golgi network had an acidic pH of 6.1, while the prevacuole and late prevacuole were both more alkaline, with pH of 6.6 and 7.1, respectively. We also showed that endosomal pH, and subsequently vacuolar trafficking of soluble proteins, requires both vacuolar-type H+ ATPase–dependent acidification as well as proton efflux mediated at least by the activity of endosomal sodium/proton NHX-type antiporters. |
| Databáze: | OpenAIRE |
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