Determination of the Main Phase Transition Temperature of Phospholipids by Nanoplasmonic Sensing
| Autor: | Wen Chen, Filip Duša, Joanna Witos, Susanne K. Wiedmer, Suvi-Katriina Ruokonen |
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| Přispěvatelé: | University of Helsinki, Czech Academy of Sciences, Department of Bioproducts and Biosystems, Aalto-yliopisto, Aalto University, Department of Chemistry, Susanne Wiedmer / Principal Investigator, Laboratory for Instruction in Swedish (-2016), Doctoral Programme in Chemistry and Molecular Sciences |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Phase transition
Materials science SUPPORTED LIPID-BILAYERS 116 Chemical sciences Analytical chemistry lcsh:Medicine 02 engineering and technology MODEL MEMBRANES 010402 general chemistry LIPOSOME 01 natural sciences Article Phase Transition DIFFERENTIAL SCANNING CALORIMETRY VESICLES Differential scanning calorimetry Phase (matter) QUARTZ-CRYSTAL MICROBALANCE Transition Temperature Lipid bilayer lcsh:Science Phospholipids Liposome Multidisciplinary DRUG-DELIVERY SYSTEMS Transition temperature Vesicle lcsh:R Biological membrane Surface Plasmon Resonance BIOLOGICAL-MEMBRANES 021001 nanoscience & nanotechnology 0104 chemical sciences NANOMATERIALS SCIENCE IONIC LIQUIDS Liposomes lcsh:Q 0210 nano-technology |
| Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018) Scientific Reports |
| Popis: | Our study demonstrates that nanoplasmonic sensing (NPS) can be utilized for the determination of the phase transition temperature (Tm) of phospholipids. During the phase transition, the lipid bilayer undergoes a conformational change. Therefore, it is presumed that the Tm of phospholipids can be determined by detecting conformational changes in liposomes. The studied lipids included 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC). Liposomes in gel phase are immobilized onto silicon dioxide sensors and the sensor cell temperature is increased until passing the Tm of the lipid. The results show that, when the system temperature approaches the Tm, a drop of the NPS signal is observed. The breakpoints in the temperatures are 22.5 °C, 41.0 °C, and 55.5 °C for DMPC, DPPC, and DSPC, respectively. These values are very close to the theoretical Tm values, i.e., 24 °C, 41.4 °C, and 55 °C for DMPC, DPPC, and DSPC, respectively. Our studies prove that the NPS methodology is a simple and valuable tool for the determination of the Tm of phospholipids. |
| Databáze: | OpenAIRE |
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