Genetic relationships of Aeromonas strains inferred from 16S rRNA, gyrB and rpoB gene sequences
| Autor: | Peter Kuhnert, Mara Küpfer, Bożena M. Korczak, Raffaele Peduzzi, Antonella Demarta |
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| Rok vydání: | 2006 |
| Předmět: |
DNA
Bacterial Sequence analysis Molecular Sequence Data Biology DNA Ribosomal Microbiology Species Specificity Phylogenetics RNA Ribosomal 16S Animals Humans Child Ribosomal DNA Phylogeny Ecology Evolution Behavior and Systematics Genetics Phylogenetic tree DNA-Directed RNA Polymerases Sequence Analysis DNA General Medicine Ribosomal RNA rpoB 16S ribosomal RNA biology.organism_classification Bacterial Typing Techniques Aeromonas DNA Gyrase Genes Bacterial Cattle |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 56:2743-2751 |
| ISSN: | 1466-5034 1466-5026 |
| Popis: | Genetic relationships among bacterial strains belonging to the genus Aeromonas were inferred from 16S rRNA, gyrB and rpoB gene sequences. Twenty-eight type or collection strains of the recognized species or subspecies and 33 Aeromonas strains isolated from human and animal specimens as well as from environmental samples were included in the study. As reported previously, the 16S rRNA gene sequence is highly conserved within the genus Aeromonas, having only limited resolution for this very tight group of species. Analysis of a 1.1 kb gyrB sequence confirmed that this gene has high resolving power, with maximal interspecies divergence of 15.2 %. Similar results were obtained by sequencing only 517 bp of the rpoB gene, which showed maximal interspecies divergence of 13 %. The topologies of the gyrB- and rpoB-derived trees were similar. The results confirm the close relationship of species within the genus Aeromonas and show that a phylogenetic approach including several genes is suitable for improving the complicated taxonomy of the genus. |
| Databáze: | OpenAIRE |
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