OPTICAL STUDIES OF SINGLE MOLECULES AT ROOM TEMPERATURE

Autor: Jay K. Trautman, X. Sunney Xie
Rok vydání: 1998
Předmět:
Zdroj: Annual Review of Physical Chemistry. 49:441-480
ISSN: 1545-1593
0066-426X
DOI: 10.1146/annurev.physchem.49.1.441
Popis: ▪ Abstract Recent developments in optical studies of single molecules at room temperature are reviewed, with an emphasis on the underlying principles and the potential of single-molecule experiments. Examples of single-molecule studies are given, including photophysics and photochemistry pertinent to single-molecule measurements, spectral fluctuations, Raman spectroscopy, diffusional motions, conformational dynamics, fluorescence resonant energy transfer, exciton dynamics, and enzymatic turnovers. These studies illustrate the information obtainable with the single-molecule approach that is hidden in ensemble-averaged measurements.
Databáze: OpenAIRE
Popis
Abstrakt:▪ Abstract Recent developments in optical studies of single molecules at room temperature are reviewed, with an emphasis on the underlying principles and the potential of single-molecule experiments. Examples of single-molecule studies are given, including photophysics and photochemistry pertinent to single-molecule measurements, spectral fluctuations, Raman spectroscopy, diffusional motions, conformational dynamics, fluorescence resonant energy transfer, exciton dynamics, and enzymatic turnovers. These studies illustrate the information obtainable with the single-molecule approach that is hidden in ensemble-averaged measurements.
ISSN:15451593
0066426X
DOI:10.1146/annurev.physchem.49.1.441