Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells
| Autor: | Ying-Jen Chen, Jia-Fan Chen, Hong-Lin Chan, Hsiu-Chuan Chou, Yen-Hung Lai, Yu-Shan Wei, Yi-Shiuan Wang, Yi-Ting Tsai, Ying-Ray Lee, Meng-Wei Lin, Mei-Lan Ko, Wen-Hung Kuo, Chih-Chun Lin |
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| Rok vydání: | 2019 |
| Předmět: |
Proteomics
Protein Folding Clinical Biochemistry Pharmaceutical Science Antineoplastic Agents 01 natural sciences Analytical Chemistry Cell Line Cell Line Tumor Drug Discovery medicine Humans Antrodia Cytotoxicity Spectroscopy Cytoskeleton biology 010405 organic chemistry Chemistry Liver cell 010401 analytical chemistry Liver Neoplasms Cancer Hep G2 Cells biology.organism_classification medicine.disease Endoplasmic Reticulum Stress 0104 chemical sciences Cell culture Cancer research Hepatocytes Liver cancer Polyporales Antrodia cinnamomea Oxidation-Reduction |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 187 |
| ISSN: | 1873-264X |
| Popis: | Antrodia Cinnamomea is a fungus species widely used as a herb medicine for hypertension, cancer and handover. Nevertheless, the biological roles of Antrodia Cinnamomea on the molecular mechanism of liver cancer are not entirely understood. To determine whether Antrodia Cinnamomea is able to be used for the treatment of liver cancer and its molecular mechanism, we examined the effect of Antrodia Cinnamomea on the differential proteomic patterns in liver cancer cell lines HepG2 and C3A as well as in Chang's liver cell, a normal liver cell, using quantitative proteomic approach. The proteomic analysis demonstrated that abundance of 82, 125 and 125 proteins was significantly altered in Chang's liver cells, C3A and HepG2, respectively. The experimental outcomes also demonstrated that Antrodia Cinnamomea-induced cytotoxicity in liver cancer cells mostly involved dysregulation of protein folding, cytoskeleton regulation, redox-regulation, glycolysis pathway as well as transcription regulation. Further analysis also revealed that Antrodia Cinnamomea promoted misfolding of intracellular proteins and dysregulate of cellular redox-balance resulting in ER-stress. To sum up our studies demonstrated that the proteomic strategy used in this study offered a tool to investigate the molecular mechanisms of Antrodia Cinnamomea-induced liver cancer cytotoxicity. The proteomic results might be further evaluated as prospective targets in liver cancer treatment. |
| Databáze: | OpenAIRE |
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