Lysophosphatidic acid enhances PGE2 to PGF2α ratio and nitric oxide level in nonpregnant buffalo uterus
| Autor: | Manickam Kesavan, Kumari Soni, T.S. Shyam Kumar, K.S. Suhas, Vivek Srivastava, Thakur Uttam Singh, Subhashree Parida, C. Gokul, Manjit Panigrahi, Archana Mahobiya |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Buffaloes Uterus Estrous Cycle Dinoprost Nitric Oxide Endometrium Dinoprostone Andrology 03 medical and health sciences chemistry.chemical_compound Food Animals Lysophosphatidic acid medicine Animals Receptors Lysophosphatidic Acid Small Animals Receptor LPAR3 LPAR1 Equine Chemistry LPAR6 030104 developmental biology medicine.anatomical_structure Cyclooxygenase 2 Female lipids (amino acids peptides and proteins) Animal Science and Zoology Lysophospholipids Corpus luteum Signal Transduction |
| Zdroj: | Theriogenology. 120:47-55 |
| ISSN: | 0093-691X |
| Popis: | Lysophosphatidic acid (LPA) is a small ubiquitous lipid exerting diverse biological functions. Its role in reproduction in different species has created great interest in recent times. In the present study, we aimed to elucidate LPA signaling in nonpregnant buffalo uterus by in vitro studies. Standard techniques like real-time PCR (for mRNA expression of LPARs and COX-2 and iNOS), Western blot (for PPARγ protein expression), sandwich ELISA (for PGE2 and PGF2α assay) and histopathology (for assessment of endometrial architecture in culture) were used in this study. The buffalo uterine tissues were collected from the local slaughterhouse and were selected for the study on the basis of the presence of corpus luteum on the ovary (n = 5). The LPAR3 receptor was the highest expressed receptor as compared to LPAR1 and LPAR6 in non-pregnant uterine tissues after 6 h incubation in Dulbecco's Modified Eagle Medium (DMEM). 50 μM LPA increased the mRNA expressions of COX-2 and iNOS enzymes which were attenuated by the treatment of LPAR1/3 antagonist Ki16425. PPARγ antagonist GW9662 prevented the LPA-induced increase in iNOS mRNA expression but did not alter the COX-2 expression. LPA also enhanced the PGE2 to PGF2α ratio in uterine tissue homogenates which was inhibited by all the receptor antagonists as well as by the inhibitors of COX-2 and iNOS. LPA also increased the total nitrite level in tissue homogenates in LPAR1/3- and iNOS-dependent manner. Additionally, we demonstrate PPARγ mRNA and protein expressions in nonpregnant buffalo endometrium. In conclusion, the results of the present study suggest that LPA acts as a luteotropic factor during the estrus cycle in nonpregnant buffalo uterus by enhancing PGE2 to PGF2α ratio and NO level through multiple receptors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect