TGF-β induced miR-132 enhances the activation of TGF-β signaling through inhibiting SMAD7 expression in glioma cells
| Autor: | Guo-Fei Li, Jian-Lei Zhang, Dong-Lin Zheng, Zhao-Hui Wang, Yan-Li Duan, Qi-Shun Zhang |
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| Rok vydání: | 2015 |
| Předmět: |
Untranslated region
Male Angiogenesis Biophysics medicine.disease_cause Biochemistry Cell Line Smad7 Protein miR-132 Transforming Growth Factor beta Glioma medicine Humans Molecular Biology Chemistry Brain Neoplasms Brain Cell Biology medicine.disease Cell biology Gene Expression Regulation Neoplastic MicroRNAs Biomarker (medicine) Female Signal transduction Carcinogenesis Transforming growth factor Signal Transduction |
| Zdroj: | Biochemical and biophysical research communications. 463(3) |
| ISSN: | 1090-2104 |
| Popis: | Transforming growth factors β (TGF-β) pathway has been proven to play important roles in oncogenesis and angiogenesis of gliomas. MiR-132 might be related to TGF-β signaling pathway and high miR-132 expression was reported to be a biomarker of poor prognosis in patients diagnosed with glioma. However, the expression regulation way involved in TGF-β pathway and clinical significance of miR-132 have not been investigated in glioma cells. Here we reported that the mRNA level of miR-132 and TGF-β concentration were both increased in patients with brain glioma. Correlation analysis revealed that TGF-β concentration was positively correlated with mRNA level of miR-132. In addition, the mRNA level of miR-132 was up-regulated by TGF-β in a concentration-dependent and time-dependent manner. Furthermore, we found that miR-132 was involved in modulation of the TGF-β signaling pathway and down-regulation of SMAD7 expression by directly targeting the SMAD7 3'-UTR. MiR-132 was negatively correlated with SMAD7 in patients with brain glioma. Taken together, our results suggest that miR-132 could be stimulated by TGF-β and might enhance the activation of TGF-β signaling through inhibiting SMAD7 expression in glioma cells. These findings contribute to a better understanding of the mechanism of the activation of TGF-β signaling by miR-132. |
| Databáze: | OpenAIRE |
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