Towards disposable lab-on-a-chip: Poly(methylmethacrylate) microchip electrophoresis device with electrochemical detection
| Autor: | Wolfgang Dörner, Alberto Escarpa, Martin Pumera, Renate Konrad, Joseph Wang, Holger Löwe, Anja Griebel, Madhu Prakash Chatrathi |
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| Rok vydání: | 2002 |
| Předmět: |
Materials science
Chromatography Microchemistry Clinical Biochemistry Detector Electrophoresis Capillary Electrochemical detection Lab-on-a-chip Electrochemistry Biochemistry Amperometry Analytical Chemistry law.invention Catecholamines Hydrazines Capillary electrophoresis Phenols Poly methylmethacrylate law Microchip Electrophoresis Polymethyl Methacrylate Disposable Equipment |
| Zdroj: | ELECTROPHORESIS. 23:596-601 |
| ISSN: | 1522-2683 0173-0835 |
| DOI: | 10.1002/1522-2683(200202)23:4<596::aid-elps596>3.0.co;2-c |
| Popis: | A fully disposable microanalytical device based on combination of poly(methylmethacrylate) (PMMA) capillary electrophoresis microchips and thick-film electrochemical detector strips is described. Variables influencing the separation efficiency and amperometric response, including separation voltage or detection potential are assessed and optimized. The versatility, simplicity and low-cost advantages of the new design are coupled to an attractive analytical performance, with good precision (relative standard deviation RSD = 1.68% for n = 10). Applicability for assays of mixtures of hydrazine, phenolic compounds, and catecholamines is demonstrated. Such coupling of low-cost PMMA-based microchips with thick-film electrochemical detectors holds great promise for mass production of single-use micrototal analytical systems. |
| Databáze: | OpenAIRE |
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