Assessment of circulating tumor cells in peripheral blood using flow cytometry in patients with surgery for colorectal cancer – review
| Autor: | Maria-Gabriela Aniţei, Iulian Radu, Andrian Panuţa, Mădălina Ştefan, Viorel Scripcariu, Bogdan Filip, Ana-Maria Muşină, Mihaela Buna-Arvinte, Mihaela Mentel, Ionuţ Huţanu, Mihaela Zlei, Dragoş Viorel Scripcariu, Mihaela-Mădălina Gavrilescu |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Colorectal cancer education colorectal cancer circulating tumor cells Flow cytometry 03 medical and health sciences 0302 clinical medicine Circulating tumor cell hemic and lymphatic diseases medicine In patient neoplasms medicine.diagnostic_test business.industry flow cytometry medicine.disease digestive system diseases Peripheral blood 030104 developmental biology 030220 oncology & carcinogenesis Medicine business |
| Zdroj: | Romanian Journal of Laboratory Medicine, Vol 28, Iss 4, Pp 365-379 (2020) |
| ISSN: | 2284-5623 |
| Popis: | Introduction: Colorectal cancer (CRC) is the third most common neoplasia in the world. Circulating tumor cells (CTC) have a prognostic value and can be useful in monitoring solid neoplasia. Only one method for CTC identification has received the approval and this is the CellSearch® system based on the immunomagnetic separation. Multiple markers are used in CTC identification, as epithelial markers and cytokeratines. CTC identification in peripheral blood is associated with a worse prognostic and reduced free survival in CRC. Material and methods: We performed a systematic search in PubMed database for articles that reports the circulating tumor cells in CRC until July 2019. We selected studies in English and French and the main words used for search were ‘circulating tumor cells’, ‘colorectal cancer’, ‘colon cancer’, ‘rectal cancer’, ‘flow cytometry’, ‘peripheral blood’. We included studies with more than 10 patients, where samples were collected from the blood in relation with surgery and flow cytometry was used as analyzing technique. Results: We included 7 studies in final analysis, that showed in flow cytometry analysis a cut-off value of CTC that can vary from 2-4 CTC/ 7.5 ml peripheral blood with a sensitivity of 50.8% and specificity of 95%. Patients with positive CTC were associated with higher T stage and positive lymph nodes, with a worse overall survival (OS) and disease free survival (DFS) comparing with negative patients. Conclusion: CTC are considered to be a prognostic factor who needs more validation studies in order to be included in the clinical practice. |
| Databáze: | OpenAIRE |
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