Differential expression and clinical significance of three inflammation-related microRNAs in gangliogliomas
| Autor: | P.C. van Rijen, J. van Scheppingen, Jasper J. Anink, Wim G.M. Spliet, A. Y N Schouten van Meeteren, Avanita S. Prabowo, Eleonora Aronica, Anand Iyer |
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| Přispěvatelé: | Cellular and Computational Neuroscience (SILS, FNWI), Faculteit der Geneeskunde, Pathology, Graduate School, Amsterdam institute for Infection and Immunity, Amsterdam Neuroscience, Cancer Center Amsterdam, Amsterdam Public Health, Paediatric Oncology |
| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Adolescent Neuroscience(all) Immunology Nerve Tissue Proteins Inflammation In situ hybridization Biology Research Support Epileptogenesis Young Adult Cellular and Molecular Neuroscience Cell Line Tumor Cortex (anatomy) microRNA Journal Article medicine Humans Non-U.S. Gov't Child Ganglioglioma miRNA Cerebral Cortex Epilepsy Brain Neoplasms Research Research Support Non-U.S. Gov't General Neuroscience Infant Middle Aged Tumor Pathology Immunohistochemistry MicroRNAs Real-time polymerase chain reactions Ki-67 Antigen Gangliogliomas medicine.anatomical_structure Neurology Cerebral cortex Child Preschool Cytokines Female medicine.symptom |
| Zdroj: | Journal of neuroinflammation, 12:97. BioMed Central Journal of Neuroinflammation Journal of Neuroinflammation [E], 12(1). BioMed Central Journal of neuroinflammation, 12(1). BioMed Central |
| ISSN: | 1742-2094 |
| DOI: | 10.1186/s12974-015-0315-7 |
| Popis: | Purpose miR21, miR146, and miR155 represent a trio of microRNAs which has been shown to play a key role in the regulation of immune and inflammatory responses. In the present study, we investigated the differential expression and clinical significance of these three miRNAs in glioneuronal tumors (gangliogliomas, GGs) which are characterized by prominent activation of the innate immune response. Methods The expression levels of miR21, miR146, and miR155 were evaluated using Taqman PCR in 34 GGs, including 15 cases with sufficient amount of perilesional cortex. Their expression was correlated with the tumor features and the clinical history of epilepsy. In addition, in situ hybridization was used to evaluate their cellular distribution in both tumor and peritumoral cortex. Results Increased expression of miR146a was observed in both tumor and peritumoral cortex compared to control samples. miR146a was detected in both neuronal and astroglial cells. Tumor and peritumoral miR146a expression was negatively correlated with frequency of seizures and the density of activated microglial cells. Neuronal and astroglial expression was observed for both miR21 and miR155 with increased expression of miR21 within the tumor and miR155 in the peritumoral region. Negative correlations were observed between the miRNA levels and the expression of putative targets within the astroglial component of the tumor. Conclusion We report a differential regulation of three miRNAs, known to be related to inflammation, in both tumor and peritumoral cortex of patients with GG. Moreover, our findings suggest a functional relationship between miR146a expression and epilepsy, either directly in epileptogenesis or as modulation of seizure activity. Electronic supplementary material The online version of this article (doi:10.1186/s12974-015-0315-7) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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