Knockdown of long non-coding RNA HOTAIR inhibits proliferation and invasiveness and improves radiosensitivity in colorectal cancer
| Autor: | Hong-Tao Xu, Wei Liu, Chungen Xing, Junjia Zhu, Gan Ru, Yongyou Wu, Ming Li, Kui Zhao, Xiaodong Yang, Shuyu Zhang, Xiaohui Xu, Yong Wu, Jianping Cao |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Cancer Research Biology medicine.disease_cause Radiation Tolerance 03 medical and health sciences 0302 clinical medicine RNA interference Cell Movement Radioresistance Cell Line Tumor medicine Humans Neoplasm Invasiveness Cell Proliferation Gene knockdown Oncogene Cancer HOTAIR General Medicine medicine.disease Prognosis digestive system diseases Long non-coding RNA Up-Regulation Gene Expression Regulation Neoplastic 030104 developmental biology Oncology 030220 oncology & carcinogenesis Gene Knockdown Techniques Cancer research RNA Long Noncoding Carcinogenesis Colorectal Neoplasms |
| Zdroj: | Oncology reports. 35(1) |
| ISSN: | 1791-2431 |
| Popis: | Colorectal cancer (CRC) is still one of the most important neoplasias causing human death. Multidisciplinary therapy has won consensus in the management of CRC, of which, radiotherapy occupies an important position. However, radioresistance is still a major obstacle in local control of CRC. Overexpression of long non-coding RNA HOTAIR has been found to correlate with tumorigenesis and poor prognosis in several types of cancer. In the present study, we analyzed HOTAIR expression levels of 53 CRC patients in tumor and adjacent normal tissue by real-time quantitative PCR. Knockdown of HOTAIR by RNA interference was performed to explore its roles in cell proliferation, migration, invasion, apoptosis and radiosensitivity. Results showed that CRC patients had higher HOTAIR expression in tumor tissues compared with adjacent normal tissues. In vitro, downregulation of HOTAIR reduced proliferation, migration and invasiveness while enhanced apoptosis and radio-sensitivity of CRC cells. Taken together, our findings suggest that long non-coding RNA HOTAIR expression is closely associated with tumor invasion and radiosensitivity, indicating the potential role in diagnostics and therapeutics of CRC. |
| Databáze: | OpenAIRE |
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