Notch-1 and Notch-2 exhibit unique patterns of expression in human B-lineage cells
| Autor: | Tucker W. LeBien, Craig E. Eckfeldt, Fred E. Bertrand, A. S. Lysholm |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Cancer Research
Stromal cell Cellular differentiation Notch signaling pathway Receptors Cell Surface Biology Mice medicine Animals Humans Cell Lineage Receptor Notch2 Receptor Notch1 Notch 2 Notch 1 B cell DNA Primers Genetics B-Lymphocytes Base Sequence Reverse Transcriptase Polymerase Chain Reaction Membrane Proteins Hematology Flow Cytometry Cell biology Mice Inbred C57BL medicine.anatomical_structure Oncology Notch proteins Hes3 signaling axis Signal Transduction Transcription Factors |
| Zdroj: | Leukemia. 14:2095-2102 |
| ISSN: | 1476-5551 0887-6924 |
| Popis: | The Notch genes encode a conserved family of receptors that influence developmental fate in many species. Prior studies have indicated that Notch-1 and Notch-2 signaling influence the development of hematopoietic stems cells and thymocytes, but little is known regarding Notch expression and function in B-lineage cells. We analyzed the expression of Notch receptors and Notch ligands in human B-lineage cells and bone marrow (BM) stromal cells. Notch-1 mRNA and protein is expressed throughout normal B cell development and in leukemic B-lineage cells. In contrast, Notch-2 expression is limited to pre-B cells expressing low levels of surface mu. The Notch ligand Delta is expressed in BM B-lineage cells. The Notch ligand Jagged-1 is not expressed in B-lineage cells, but is expressed in BM stromal cells. These results suggest a model wherein lateral signaling between Notch and Delta on B-lineage cells and/or Notch/Jagged-1 interactions between B-lineage cells and BM stromal cells may regulate human B cell development. |
| Databáze: | OpenAIRE |
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