Cinématique de réorientation de E. coli à proximité d'une surface solide
| Autor: | Cao Cuong Nguyen, Christophe Place, Cédric Vaillant, Elodie Chatre, T. Cajgfinger, Jean-François Palierne, Laurence Lemelle, Agnes Dominjon, Remi Barbier |
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| Přispěvatelé: | Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement (LGL-TPE), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut national des sciences de l'Univers (INSU - CNRS)-Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS), Institut de Physique des 2 Infinis de Lyon (IP2I Lyon), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique de l'ENS Lyon (Phys-ENS), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement [Lyon] (LGL-TPE), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Claude Bernard Lyon 1 (UCBL), École normale supérieure - Lyon (ENS Lyon)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Surface (mathematics)
Physics 0303 health sciences Models Statistical High magnification Chemotaxis Solid surface [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] Biophysics Geometry Kinematics Thermal diffusivity Models Biological [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Article Biomechanical Phenomena 03 medical and health sciences 0302 clinical medicine Flagella 13. Climate action Escherichia coli High temporal resolution Clockwise 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | Biophysical Journal Biophysical Journal, 2020, 118 (10), pp.2400-2410. ⟨10.1016/j.bpj.2020.03.024⟩ Biophysical Journal, Biophysical Society, 2020, 118 (10), pp.2400-2410. ⟨10.1016/j.bpj.2020.03.024⟩ Biophys J |
| ISSN: | 0006-3495 1542-0086 |
| Popis: | International audience; Bacteria tumble periodically, following environmental cues. Whether they can tumble near a solid surface is a basic issue for the inception of infection or mineral biofouling. Observing freely swimming Escherichia coli near and parallel to a glass surface imaged at high magnification (Â100) and high temporal resolution (500 Hz), we identified tumbles as events starting (or finishing, respectively) in abrupt deceleration (or reacceleration, respectively) of the body motion. Selected events show an equiprobable clockwise (CW) or counterclockwise change in direction that is superimposed on a surface CW path because of persistent propulsion. These tumbles follow a common long (about 300 5 100 ms, N ¼ 52) deceleration-reorientation acceleration pattern. A wavelet transform multiscale analysis shows these tumbles cause in-plane diffusive reorientations with 1.5 rad 2 /s rotational diffusivity, a value that compares with that measured in bulk tumbles. In half of the cases, additional few-millisecond bursts of an almost equiprobable CW or counterclockwise change of direction (12 5 90 , N ¼ 89) occur within the reorientation stage. The highly dispersed absolute values of change of direction (70 5 66 , N ¼ 89) of only a few bursts destabilize the cell-swimming direction. These first observations of surface tumbles set a foundation for statistical models of run-and-tumble surface motion different from that in bulk and lend support for chemotaxis near solid surface. |
| Databáze: | OpenAIRE |
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