Spatiotemporal material functionalization via competitive supramolecular complexation of avidin and biotin analogs
| Autor: | Marcel Karperien, Michelle Koerselman, Jan Hendriks, Tom Kamperman, Xandra de Peuter, Cindy Kelder, João F. Crispim, Jeroen Leijten, Pieter J. Dijkstra |
|---|---|
| Přispěvatelé: | Orthopaedic Biomechanics, Developmental BioEngineering |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Macromolecular Substances
Science Supramolecular chemistry Biotin General Physics and Astronomy Biocompatible Materials Nanotechnology 02 engineering and technology 010402 general chemistry 01 natural sciences Antibodies Article General Biochemistry Genetics and Molecular Biology Cell Line Mice chemistry.chemical_compound Spatio-Temporal Analysis Animals Humans Biotinylation lcsh:Science Multidisciplinary Tissue Engineering biology Bioinspired materials Biomaterial Hydrogels NeutrAvidin General Chemistry Avidin 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Drug delivery Self-healing hydrogels biology.protein Nanoparticles Surface modification lcsh:Q Biomaterials - cells Peptides 0210 nano-technology |
| Zdroj: | Nature Communications, Vol 10, Iss 1, Pp 1-11 (2019) Nature Communications, 10:4347. Nature Publishing Group Nature Communications Nature communications, 10(1):4347. Nature Publishing Group |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-019-12390-4 |
| Popis: | Spatiotemporal control over engineered tissues is highly desirable for various biomedical applications as it emulates the dynamic behavior of natural tissues. Current spatiotemporal biomaterial functionalization approaches are based on cytotoxic, technically challenging, or non-scalable chemistries, which has hampered their widespread usage. Here we report a strategy to spatiotemporally functionalize (bio)materials based on competitive supramolecular complexation of avidin and biotin analogs. Specifically, an injectable hydrogel is orthogonally post-functionalized with desthiobiotinylated moieties using multivalent neutravidin. In situ exchange of desthiobiotin by biotin enables spatiotemporal material functionalization as demonstrated by the formation of long-range, conformal, and contra-directional biochemical gradients within complex-shaped 3D hydrogels. Temporal control over engineered tissue biochemistry is further demonstrated by timed presentation and sequestration of growth factors using desthiobiotinylated antibodies. The method’s universality is confirmed by modifying hydrogels with biotinylated fluorophores, peptides, nanoparticles, enzymes, and antibodies. Overall, this work provides a facile, cytocompatible, and universal strategy to spatiotemporally functionalize materials. Controlled patterning of functionality within hydrogels typically involves complex chemistry. Here, the authors report on a simple competitive binding strategy using avidin and biotin analogs in an injectable biomaterial for spatiotemporally controlled presentation of biochemical stimuli to cells. |
| Databáze: | OpenAIRE |
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