Isolation and Characterization of a Moderately Thermophilic Anaerobic Alkaliphile, Clostridium paradoxum sp. nov

Autor:	Linda Mandelco, Juergen Wiegel, Youhong Li
Rok vydání:	1993
Předmět:	Thermophile Immunology Maltose Biology biology.organism_classification Microbiology chemistry.chemical_compound Clostridium chemistry Dissimilatory sulfate reduction Tryptone Alkaliphile Anaerobic bacteria Bacteria
Zdroj:	Scopus-Elsevier
ISSN:	1465-2102 0020-7713
DOI:	10.1099/00207713-43-3-450
Popis:	Alkaliphilic, moderately thermophilic anaerobic bacteria able to grow above pH 10.5 and 55°C were isolated from various sewage plants in the United States. The strains were motile with two to six peritrichous flagella and formed round to slightly oval terminal spores in terminally distended and slightly enlarged cells. Sporulated cells remained motile. The pH range for growth was between 7.0 and 11.1, with an optimum of around 10.1 At pH 10.1 the temperature range for growth was between 30 and 63°C, with an optimum of 56°C. The shortest observed doubling time (glucose) was around 16 min at 56°C and pH 10.1. No dissimilatory sulfate reduction was detected. The organism utilized glucose, fructose, sucrose, maltose, and pyruvate but required yeast extract or tryptone for growth. Optimal NaCI concentrations for growth were between 50 and 200 mM. The guanine-plus-cytosine content was 30.0 ± 0.10 mol%. On the basis of unique properties and 16S rRNA analysis, the strains are placed in a new species, Clostridium paradoxum, referring to the unusual retainment of motility by sporulated cells. Strain JW-YL-7 (DSM 7308) is designated as the type strain.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ca430c6b0b5ec4674cd04f63523d996 https://doi.org/10.1099/00207713-43-3-450 Zobrazit plný text záznamu