Circulating Ki-67 index in plasma as a biomarker and prognostic indicator in chronic lymphocytic leukemia
| Autor: | Heather R. Sanders, Wanlong Ma, Hagop M. Kantarjian, Amber C. Donahue, Chen-Hsiung Yeh, Jean Marie Bruey, Zeev Estrov, Maher Albitar, Susan O'Brien, Michael J. Keating |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Cancer Research medicine.medical_specialty Chronic lymphocytic leukemia Blotting Western Gastroenterology Article Jurkat Cells Internal medicine medicine Humans Lymph node Aged Proportional Hazards Models Aged 80 and over biology Proportional hazards model Beta-2 microglobulin Hematology Middle Aged medicine.disease Prognosis Immunohistochemistry Leukemia Lymphocytic Chronic B-Cell Leukemia medicine.anatomical_structure Ki-67 Antigen Oncology Ki-67 Immunology biology.protein Biomarker (medicine) Female beta 2-Microglobulin Biomarkers |
| Zdroj: | Leukemia research. 34(10) |
| ISSN: | 1873-5835 |
| Popis: | Ki-67 is a nuclear antigen that is expressed in all stages of the cell cycle, except G(0), and is widely used as a marker of cellular proliferation in human tumors. We recently showed that elevated levels of Ki-67 circulating in plasma (cKi-67) are associated with shorter survival in patients with acute lymphoblastic leukemia. The current study included 194 patients with CLL and 96 healthy control subjects. cKi-67 levels in plasma were determined using an electrochemiluminescent immunoassay. We normalized the cKi-67 level to the absolute number of lymphocytes in the patient's peripheral blood to establish the plasma cKi-67 index. The cKi-67 index showed significant correlation with lymph node involvement and Rai stage (P=0.05). Higher cKi-67 index values were significantly associated with shorter survival. Multivariate Cox proportional hazards regression analysis demonstrated that the association of the cKi-67 index with shorter survival was independent of IgV(H) mutation status. In a multivariate model incorporating the cKi-67 index with B2M and IgV(H), only cKi-67 index and B2M levels remained as independent predictors of survival. The results of this study suggest that the plasma cKi-67 index, along with B2M level, is a strong predictor of clinical behavior in CLL. |
| Databáze: | OpenAIRE |
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