Acellular derivatives of mesenchymal stem cells prevent peritoneal adhesions in an animal model
Autor: | Paulette Conget, Daniel Rojo |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty medicine.medical_treatment Tissue Adhesions Inflammation Mesenchymal Stem Cell Transplantation Peritoneal Diseases Fibrin Proinflammatory cytokine Mice 03 medical and health sciences 0302 clinical medicine Fibrosis Fibrinolysis medicine Animals biology business.industry Mesenchymal stem cell medicine.disease Disease Models Animal 030104 developmental biology 030220 oncology & carcinogenesis biology.protein Cytokines Female Surgery Tumor necrosis factor alpha medicine.symptom business Infiltration (medical) |
Zdroj: | Journal of Surgical Research. 223:198-206 |
ISSN: | 0022-4804 |
Popis: | Background Peritoneal adhesions are nonanatomical connections that bind organs to the abdominal wall or among them. They arise after peritoneal injury, which triggers an inflammatory response followed by a healing process that leads to fibrotic tissue formation. Mesenchymal stem cells and their secretion products, also referred to as acellular derivatives (ACDs), have anti-inflammatory, fibrinolytic, and antifibrogenic properties. The aim of this study was to determine the effect of intraoperative administration of ACD on the appearance, severity, and progression of peritoneal adhesions, in an animal model. Materials and methods Cecal erosions were mechanically induced in adult mice. Before closure, the vehicle, ACD, or Seprafilm was administered. Seven days later, the presence and grade of peritoneal adhesions were assessed macroscopically. One, 3, and 7 d after intervention, molecular and cellular markers of inflammation, fibrinolysis, and fibrogenesis were evaluated both locally and systemically. Results ACDs avoided the appearance of clinically relevant peritoneal adhesions. The vehicle had no effect, and Seprafilm reduced them inconsistently. The antiadhesive effect of ACD was associated with an early reduction of proinflammatory cytokine (tumor necrosis factor-alpha and interferon-gamma) secretion and leukocyte (polymorphonuclears, mononuclears, and macrophages) infiltration. High levels of D-dimer, low fibrin deposits, low myofibroblasts infiltration, and less fibrosis were also observed. Conclusions ACD administered at the end of abdominal surgeries prevents the formation of peritoneal adhesions due to the modulation of inflammatory, fibrinolytic, and fibrogenic processes. |
Databáze: | OpenAIRE |
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