High resolution 3T fMRI in anesthetized monkeys
| Autor: | Bernard Bioulac, Franck Lamberton, Christian E. Gross, Thomas Boraud, Romaric Saulnier, Bernard Mazoyer, Alan R. Young, Palma Pro-Sistiaga |
|---|---|
| Přispěvatelé: | Institut des Maladies Neurodégénératives [Bordeaux] (IMN), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), GIP Cyceron (Cyceron), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Sérine protéases et physiopathologie de l'unité neurovasculaire, Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Groupe d'Imagerie Neurofonctionnelle (GIN - UMR 5296), Service NEUROSPIN (NEUROSPIN), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-Service NEUROSPIN (NEUROSPIN), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Baufreton, Jérôme, Hal, GIN |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
MESH: Echo-Planar Imaging Photic Stimulation [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology Signal-To-Noise Ratio Macaque MESH: Magnetic Resonance Imaging Stereotaxic Techniques Image Processing Computer-Assisted MESH: Animals Anesthesia Visual Cortex Echo-planar imaging Visual stimulation biology Echo-Planar Imaging General Neuroscience Magnetic Resonance Imaging MESH: Image Processing Computer-Assisted MESH: Atracurium MESH: Reproducibility of Results medicine.anatomical_structure MESH: Photic Stimulation Anesthetics Inhalation Atracurium MESH: Oxygen medicine.drug Methyl Ethers MESH: Stereotaxic Techniques Image processing MESH: Anesthesia MESH: Macaca mulatta 3 T magnet Sevoflurane MESH: Methyl Ethers biology.animal medicine Animals MESH: Signal-To-Noise Ratio business.industry [SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology MESH: Visual Cortex Methodology Reproducibility of Results Macaca mulatta MESH: Male Oxygen Primary sensory areas Functional imaging Stereotaxic technique Anesthetic Feasibility Studies business MESH: Neuromuscular Nondepolarizing Agents MESH: Feasibility Studies Neuroscience MESH: Anesthetics Inhalation Neuromuscular Nondepolarizing Agents BOLD |
| Zdroj: | Journal of Neuroscience Methods Journal of Neuroscience Methods, 2012, 205, pp.86-95 Journal of Neuroscience Methods, 2012, 205 (1), pp.86-95 Journal of Neuroscience Methods, Elsevier, 2012, 205 (1), pp.86-95 Journal of Neuroscience Methods, Elsevier, 2012, 205, pp.86-95 |
| ISSN: | 0165-0270 |
| Popis: | International audience; Although there are numerous 3 T MRI research devices all over the world, only a few functional studies at 3 T have been done in anesthetized monkeys. In the past, anesthetized preparations were reported to be misleading when exploring cortical brain regions outside the primary sensory areas. Nonetheless, a great improvement has been achieved in the limited effect of anesthetic agents on the reactivity of the brain.Here, we re-address the feasibility and potential applications of the brain oxygen level dependent (BOLD) fMRI signal in Macaca mulatta monkeys that have been lightly anesthetized with sevoflurane and curarized. The monkeys were studied with commercially available coils and sequences using a 3 T clinical magnet. We obtained sagittal T1 scout images, gray matter double inversion recovery, standard gradient echo sequences and gradient echo functional imaging sequences. Given that fMRI signals are most readily identified in the cerebral cortices, we optimized Echo Planar Imaging sequences to reproduce significant changes in the BOLD signal subsequent to a visual stimulation paradigm.Our results provide a satisfactory signal to noise ratio with a limited standard deviation range, when compared with studies on alert macaques.We suggest that the 3 T magnet remains a valuable tool to analyze neural pathways in the macaque brain under light anesthesia and report the use of spatially resolved fMRI in higher visual areas of anesthetized monkeys. This methodology avoids the need for time-consuming training of awake monkeys, is stable over many hours, provides reproducible data and could be applied successfully to future functional studies. |
| Databáze: | OpenAIRE |
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