Chitinolytic actinobacteria isolated from an Algerian semi-arid soil: development of an antifungal chitinase-dependent assay and GH18 chitinase gene identification
| Autor: | Hasna Boubakri, Petar Pujic, Meriem Gasmi, Philippe Normand, Lorena Carro, M. Kitouni |
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| Přispěvatelé: | Laboratoire de Biologie Appliquée et Santé, Université des Frères Mentouri (Constantine 1), Laboratoire de Génie Microbiologie et Application, Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Ministry of Higher Education and Scientific Research, Algeria, Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL) |
| Předmět: |
0106 biological sciences
Catabolic repression [SDV]Life Sciences [q-bio] Microorganism Micromonospora 01 natural sciences Applied Microbiology and Biotechnology Streptomyces Microbiology Actinobacteria Gene capture 03 medical and health sciences chemistry.chemical_compound Chitin Microbial ecology 010608 biotechnology antifungal agent Antifungal activity genes BOX-PCR 0303 health sciences GH18 biology 030306 microbiology gène Chitinase Ribosomal RNA biology.organism_classification chemistry biology.protein antifongique |
| Zdroj: | Europe PubMed Central Annals of Microbiology Annals of Microbiology, Springer, 2019, 69 (4), pp.395-405. ⟨10.1007/s13213-018-1426-z⟩ |
| ISSN: | 1590-4261 1869-2044 |
| Popis: | The purpose of this study was to explore the microbial potential of a semi-arid sandy soil from south-central Algeria in order to isolate new chitinolytic actinobacteria. This soil is subjected to high temperatures (up to 43 degrees C) and has low nutrient content. Strains were isolated by plating soil suspensions on Bennett and Colloidal Chitin (CCM) medium. An initial clustering of isolates was made through BOX-PCR genetic profiling. Next, a 16S rRNA gene sequencing of representative isolates was realized. We also identified optimum physicochemical conditions for chitinolytic activity. A rapid in vitro assay based on glucose catabolic repression was developed to select isolates having a chitinase-dependent antifungal activity against two phytopathogenic fungi. Gene identification of glycosyl hydrolase family 18 (GH18) permitted us to assess the divergence of chitinase genes. Forty isolates were obtained from the semi-arid sandy soil. The molecular identification permitted us to assign them to Streptomyces or Micromonospora genera with seven possibly new bacterial species. For chitinolytic activity, 100% of isolates were able to grow and degrade colloidal chitin at pH 7 and at a temperature ranging from 30 to 40 degrees C. We also observed that Micromonospora strains had atypical activity patterns, with a strong chitinase activity maintained at high temperature. Finally, three strains presented an interesting chitinolytic potential to reduce fungal growth with new GH18 sequences. This study presents a new rapid method to detect antifungal chitinase-dependent activity that allowed to identify potentially new species of actinobacteria and new GH18 gene sequences. |
| Databáze: | OpenAIRE |
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