Calorie restriction during gestation affects ovarian reserve in offspring in the mouse
| Autor: | Renata Pereira Ramirez, Augusto Schneider, Gabriel Barreto Veiga, Tatiana D. Saccon, Bianka M Zanini, Michal M. Masternak, Driele N Garcia, Rafael Gianella Mondadori, Luis A. X. Cruz, Carlos Castilho Barros, Joao Alveiro Alvarado-Rincón, Kelvin R. S. Andrade, Jorgea Pradieé |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Offspring Calorie restriction Reproductive technology Biology Andrology 03 medical and health sciences Mice 0302 clinical medicine Endocrinology Pregnancy Genetics medicine Animals Ovarian follicle Ovarian reserve Ovarian Reserve Molecular Biology Prenatal Nutritional Physiological Phenomena 030304 developmental biology Caloric Restriction 0303 health sciences 030219 obstetrics & reproductive medicine Reproduction Malnutrition medicine.disease Mice Inbred C57BL medicine.anatomical_structure Glucose Phenotype Reproductive Medicine Animals Newborn Prenatal Exposure Delayed Effects Gestation Animal Science and Zoology Female Folliculogenesis Developmental Biology Biotechnology |
| Zdroj: | Reproduction, fertility, and development. 32(18) |
| ISSN: | 1031-3613 |
| Popis: | The aim of this study was to investigate the effect of calorie restriction (CR) during pregnancy in mice on metabolism and ovarian function in the offspring. Pregnant female mice were divided into two groups, a control group and a CR group (n=7 in each). Mice in the CR group were fed 50% of the amount consumed by control females from Day 10 of gestation until delivery. After weaning, the offspring received diet ad libitum until 3 months of age, when ovaries were collected. Ovaries were serially cut and every sixth section was used for follicle counting. Female offspring from CR dams tended to have increased bodyweight compared with offspring from control females (P=0.08). Interestingly, fewer primordial follicles (60% reduction; P=0.001), transitional follicles (P=0.0006) and total follicles (P=0.006) were observed in offspring from CR mothers. The number of primary, secondary and tertiary follicles did not differ between the groups (P>0.05). The CR offspring had fewer DNA double-strand breaks in primary follicle oocytes (P=0.03). In summary, CR during the second half of gestation decreased primordial ovarian follicle reserve in female offspring. These findings suggest that undernutrition during the second half of gestation may decrease the reproductive lifespan of female offspring. |
| Databáze: | OpenAIRE |
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