Highly pathogenic porcine reproductive and respiratory syndrome virus GP5 B antigenic region is not a neutralizing antigenic region
| Autor: | Da-Qing Gong, Zhi-Jun Tian, Zhen-Xiang Meng, Juan-Juan Guo, Jiang Wu, Yu-Quan Wu, Chaoliang Leng, Yong-Qian Yang, Tong-Qing An, Yi Zhang, Guangzhi Tong, Deng-Yun Li, Jiazeng Chen, Jin-Mei Peng |
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| Rok vydání: | 2011 |
| Předmět: |
China
Swine viruses animal diseases Recombinant Fusion Proteins Porcine Reproductive and Respiratory Syndrome Enzyme-Linked Immunosorbent Assay Immunofluorescence Microbiology Neutralization Viral Proteins Antigen medicine Animals Porcine respiratory and reproductive syndrome virus Neutralizing antibody Antiserum General Veterinary biology medicine.diagnostic_test virus diseases Viral Vaccines General Medicine respiratory system Porcine reproductive and respiratory syndrome virus biology.organism_classification Virology Antibodies Neutralizing Titer biology.protein Rabbits Antibody Peptides |
| Zdroj: | Veterinary microbiology. 159(3-4) |
| ISSN: | 1873-2542 |
| Popis: | In 2006, highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) caused great economic losses emerged in China and continues to be a threat for the pig industry. B antigenic region (AR) ((37)SHL/FQLIYNL(45)) of GP5 was considered to be a major linear neutralizing AR in PRRSV classical strains. However, peptide-purified antibodies against this AR did not neutralize PRRSV in a recent report. Compared with classical PRRSV, one amino acid mutation (L/F(39)→ I(39)) was found in B AR of HP-PRRSV. To study the ability of B AR of HP-PRRSV to induce neutralizing antibody (NA) in vitro and in vivo, rabbit antisera against B AR with and without the mutation and pig hyperimmune sera with high titer of NAs against HP-PRRSV were prepared. Immunofluorescence assays (IFA) showed that the two rabbit antisera both had reactivity to classical PRRSV CH-1a and HP-PRRSV HuN4 with no observable difference in IFA titer. However, antisera did not have neutralizing activity against classical PRRSV CH-1a and HP-PRRSV HuN4. No correlation was observed between the levels of anti-B AR peptide antibodies and NAs in pig hyperimmune sera that were detected by indirect ELISA and virus neutralization, respectively. B AR peptide-specific serum antibodies had no neutralizing activity and, GST-B fusion protein could not inhibit neutralization of NAs in pig hyperimmune sera. Based on these findings, we conclude that B AR of HP-PRRSV is not a neutralizing AR of HP-PRRSV GP5. |
| Databáze: | OpenAIRE |
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