Efficacy and Safety of Ultrapure Alginate-Based Anti-Adhesion Gel in Experimental Peritonitis
| Autor: | Roger M. L. M. Lomme, O.R. Buyne, Thijs Hendriks, Ankit A. Chaturvedi, H. van Goor |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Microbiology (medical) Abdominal Abscess Alginates Bacterial Peritonitis Peritonitis Tissue Adhesions medicine.disease_cause Microbiology Glucuronic Acid medicine Animals Rats Wistar Abscess Escherichia coli Feces biology business.industry Hexuronic Acids Body Weight Adhesion Adhesion barrier biology.organism_classification medicine.disease Rats Reconstructive and regenerative medicine Radboud Institute for Health Sciences [Radboudumc 10] Disease Models Animal Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10] Infectious Diseases Surgery Bacteroides fragilis business Gels |
| Zdroj: | Surgical Infections, 16, 410-4 Surgical Infections, 16, 4, pp. 410-4 |
| ISSN: | 1557-8674 1096-2964 |
| DOI: | 10.1089/sur.2014.111 |
| Popis: | Item does not contain fulltext BACKGROUND: Intra-abdominal infection may lead to adhesion and abscess formation. An adhesion barrier can reduce these complications but also aggravate intra-peritoneal infection, causing the opposite effects. The fear of infection propagation has limited clinical adhesion barrier use in a contaminated or infected abdomen. This study evaluated both adhesion and abscess reduction and infection propagation of a new ultrapure alginate-based anti-adhesive barrier gel in a rat peritonitis model. METHODS: In 64 male Wistar rats, bacterial peritonitis was induced via intra-abdominal injection of a mixture of sterile feces, 10(5) colony-forming units (CFU) of Escherichia coli, and 10(4) CFU of Bacteroides fragilis. Surgical debridement and peritoneal lavage were performed 1 h after inoculation. Animals were randomly allocated in equal numbers to a control group or an alginate gel group. Animals were sacrificed on day five post-operatively. Death and the presence and size of intra-abdominal abscesses were noted, and adhesions were scored. All outcomes were compared in the two groups. RESULTS: Seventeen rats (27%) died prematurely without any difference between the groups. Of the surviving rats in the alginate gel group, 88% developed abscesses vs. 100% of the control group. There was no significant difference in the abscess scores or incidence rates of adhesion formation between the groups. The adhesion scores were lower for the alginate gel group compared with control animals (p=0.04). CONCLUSION: Ultrapure alginate gel reduces adhesion severity but not abscesses. The gel seemed to be safe, not aggravating intra-peritoneal infection in this abdominal infection model. |
| Databáze: | OpenAIRE |
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