Heterotypic interaction of fibroblasts and endothelial cells on restricted area
| Autor: | R. Barbucci, C. Di Canio, Stefania Lamponi, M. Forbicioni |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Cytoplasm
Spectrophotometry Infrared Scanning electron microscope Stereochemistry Biomedical Engineering Cell Count Microscopy Atomic Force Mass Spectrometry Cell Line Biomaterials Tissue engineering Cell–cell interaction Spectroscopy Fourier Transform Infrared Microscopy Cell Adhesion Humans Hyaluronic Acid Cell adhesion Cell Shape Cells Cultured Cell Nucleus Microscopy Video Chemistry Metals and Alloys Endothelial Cells Fibroblasts Coculture Techniques Endothelial stem cell Secondary ion mass spectrometry Attenuated total reflection Microscopy Electron Scanning Ceramics and Composites Biophysics Glass |
| Popis: | The polysaccharide hyaluronic acid (Hyal) was photoimmobilized on glass surfaces to obtain a pattern with squares and rectangles of different dimensions and chemistry. The microstructured surfaces were characterized by Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM), Attenuated Total Reflection Infrared Spectroscopy (ATR FT-IR), and Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS). Surface analysis revealed the presence of a pattern consisting of alternating glass and Hyal microstructures whose dimensions decreased from the center to the edge of the sample. The behavior of Human Coronary Artery Endothelial Cells (HCAEC) and human tumoral dermal fibroblasts (C54) was studied on these micropatterned surfaces. Neither HCAEC nor C54 adhered to the immobilized Hyal but both adapted their shape to the different sizes of the glass squares and rectangles. The number of adherent HCAEC and C54 depended on the dimensions of both the glass domains and the nuclei of the cells. Co-cultured C54 on HCAEC patterned surfaces showed a heterotypic cell-cell interaction in the same chemical and topographic domain for the first time. In comparison to other techniques for patterning two different cell types, our approach was non cytotoxic and allowed arbitrary geometric patterns to form on different biocompatible substrata. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|