The Effect of Thymoquinone, α7 Receptor Agonist and α7 Receptor Allosteric Modulator on the Cerebral Cortex in Experimentally Induced Alzheimer’s Disease in Relation to MSCs Activation
| Autor: | Osama E, Aal La, Maha Baligh Zickri, Ibrahim AbdEl Fattah L, Heikal O |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Agonist medicine.medical_specialty Allosteric modulator LPS medicine.drug_class medicine.medical_treatment Intraperitoneal injection Allosteric regulation MSCs Pharmacology 03 medical and health sciences chemistry.chemical_compound Internal medicine medicine Thymoquinone Receptor PNU- 120596 business.industry Cell Biology PNU- 282987 030104 developmental biology Anticonvulsant Nicotinic agonist Endocrinology chemistry Original Article business Alzheimer’s disease Developmental Biology |
| Zdroj: | International Journal of Stem Cells |
| ISSN: | 2005-5447 2005-3606 |
| Popis: | Background and Objectives Alzheimer's disease (AD) is the most common form of dementia among older persons. Thymoquinone (TQ) has anti-inflammatory, anticonvulsant and antioxidant activity. A novel α7 nicotinic acetyl choline receptor (α7 nAChR ) agonist (PNU- 282987) have been identified to enhance the cognitive performance. An alternative treatment strategy via compounds known as nicotinic "positive allosteric modulators" (PAMs) has been reported. This study was designed to investigate the combination of PAM of α7 nAChRs with PNU- 282987 or with TQ as a possible treatment for AD in rat. Methods 48 male albino rats were divided into 4 groups. Group І (Control), Group II received lipopolysaccharide, 0.8 mg/kg by intraperitoneal injection (IPI) once, Group III received TQ 10 mg/kg by IPI, Group IV received PNU-120596 1 mg/kg by IPI, in addition to PNU-282987 1 mg/kg by IPI in subgroup IVa and TQ in subgroup b. All treatment drugs were given for 5 days. Results Acidophilic masses, deformed neurons, Congo red +ve masses and reduced Phospho-CREB immunoexpression were seen in group II. All changes regressed by treatment. Some CD44 +ve cells were noticed in group II and few +ve cells in subgroup IVa, that became multiple in group III and subgroup IVb. The histological, histochemical and immunohistochemical changes were confirmed statistically and significant differences were recorded. Conclusions TQ or α7 nAChR agonist combined with PAM can have an important role in treatment of AD that is superior to thymoquinone alone. Exceptionally, TQ single or combined with PAM proved activation of MSC. |
| Databáze: | OpenAIRE |
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