UCE6, a New Antitumor Antibiotic with Topoisomerase I-mediated DNA Cleavage Activity Produced by Actinomycetes: Producing Organism,Fermentation, Isolation and Biological Activity
| Autor: | Tadashi Ashizawa, Noboru Fujii, Hirofumi Nakano, Futoshi Tanaka, Yoshinori Yamashita, Shigeru Chiba |
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| Rok vydání: | 1997 |
| Předmět: |
medicine.drug_class
Stereochemistry Antibiotics Neoplasms Drug Discovery Tumor Cells Cultured medicine Actinomyces Humans Pharmacology chemistry.chemical_classification Antibiotics Antineoplastic biology Chemistry Topoisomerase Quinones Biological activity DNA Neoplasm biology.organism_classification In vitro Enzyme DNA Topoisomerases Type I Biochemistry Fermentation biology.protein Bacteria Camptothecin HeLa Cells medicine.drug |
| Zdroj: | The Journal of Antibiotics. 50:490-495 |
| ISSN: | 1881-1469 0021-8820 |
| DOI: | 10.7164/antibiotics.50.490 |
| Popis: | A novel antitumor antibiotic, UCE6 (1,3,8,10,11-pentahydroxy-2-methyl-10-(2-oxo-4-hydroxypentyl)na phthacene-5, 12-dione) with topoisomerase I-mediated DNA cleavage activity, was isolated from the culture broth of actinomycetes strain UOE6. Addition of silicone oil antifoam agent, KS69 (2%), to the fermentation enhanced the production of UCE6 by approximately 3 fold. A total of 1.15 g of UCE6 was recovered as reddish orange crystals from a 100 liter fermentation supplemented with 2% KS69. UCE6 exhibited growth inhibitory activity against HeLa S3, HCT116 and Lu-65 cells comparable to that of camptothecin. |
| Databáze: | OpenAIRE |
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