Rh-I-UEA-1 polymerized liposomes target and image adenomatous polyps in the APCMin/+ mouse using optical colonography
| Autor: | Biying Xu, Jeremiah Wierwille, Shuai Yuan, Ronald M. Summers, Chao-Wei Chen, Jianwu Xie, Yu Chen, Gary L. Griffiths, Celeste A. Roney |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Pathology
medicine.medical_specialty lcsh:Medical technology Adenomatous polyposis coli Colorectal cancer Colon Adenomatous Polyposis Coli Protein Biomedical Engineering Article Adenomatous Polyps Mice Agglutinin medicine Animals Radiology Nuclear Medicine and imaging lcsh:QH301-705.5 Liposome biology Mucin Lectin Condensed Matter Physics medicine.disease Molecular biology Mice Inbred C57BL lcsh:Biology (General) lcsh:R855-855.5 Adenomatous Polyposis Coli Liposomes biology.protein Molecular Medicine Iohexol Molecular imaging Plant Lectins Colorectal Neoplasms Colonography Computed Tomographic Biotechnology medicine.drug |
| Zdroj: | Molecular Imaging, Vol 10 (2011) |
| Popis: | Mutated adenomatous polyposis coli ( APC ) genes predispose transformations to neoplasia, progressing to colorectal carcinoma. Early detection facilitates clinical management and therapy. Novel lectin-mediated polymerized targeted liposomes (Rh-I-UEA-1), with polyp specificity and incorporated imaging agents were fabricated to locate and image adenomatous polyps in APC Min /+ mice. The biomarker α- l -fucose covalently joins the liposomal conjugated lectin Ulex europaeus agglutinin (UEA-1), via glycosidic linkage to the polyp mucin layer. Multispectral optical imaging (MSI) corroborated a global perspective of specific binding (rhodamine B 532 nm emission, 590–620 nm excitation) of targeted Rh-I-UEA-1 polymerized liposomes to polyps with 1.4-fold labeling efficiency. High-resolution coregistered optical coherence tomography (OCT) and fluorescence molecular imaging (FMI) reveal the spatial correlation of contrast distribution and tissue morphology. Freshly excised APC Min bowels were incubated with targeted liposomes (UEA-1 lectin), control liposomes (no lectin), or iohexol (Omnipaque) and imaged by the three techniques. Computed tomographic quantitative analyses did not confirm that targeted liposomes more strongly bound polyps than nontargeted liposomes or iohexol (Omnipaque) alone. OCT, with anatomic depth capabilities, along with the coregistered FMI, substantiated Rh-I-UEA-1 liposome binding along the mucinous polyp surface. UEA-1 lectin denotes α- l -fucose biomarker carbohydrate expression at the mucin glycoprotein layer; Rh-I-UEA-1 polymerized liposomes target and image adenomatous polyps in APC Min mice. |
| Databáze: | OpenAIRE |
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