Effect of Artemisinin-Loaded Mesoporous Cerium-Doped Calcium Silicate Nanopowder on Cell Proliferation of Human Periodontal Ligament Fibroblasts
| Autor: | Anna Theocharidou, Dimitrios Gkiliopoulos, Karine Reybier, Pierre Perio, Georgia K. Pouroutzidou, Konstantinos M. Paraskevopoulos, Eleana Kontonasaki, Chrysanthi Papoulia, Evgenia Lymperaki, Ioannis Tsamesidis |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
inorganic chemicals
Biocompatibility Scanning electron microscope General Chemical Engineering technology industry and agriculture chemistry.chemical_element mesoporous nanopowders hemocompatibility Article chemistry.chemical_compound Cerium Chemistry chemistry human periodontal ligament fibroblasts Calcium silicate General Materials Science MTT assay cerium doping Fourier transform infrared spectroscopy artemisinin loading Drug carrier Mesoporous material QD1-999 Nuclear chemistry |
| Zdroj: | Nanomaterials, Vol 11, Iss 2189, p 2189 (2021) Nanomaterials Volume 11 Issue 9 |
| ISSN: | 2079-4991 |
| Popis: | Ion doping has rendered mesoporous structures important materials in the field of tissue engineering, as apart from drug carriers, they can additionally serve as regenerative materials. The purpose of the present study was the synthesis, characterization and evaluation of the effect of artemisinin (ART)-loaded cerium-doped mesoporous calcium silicate nanopowders (NPs) on the hemocompatibility and cell proliferation of human periodontal ligament fibroblasts (hPDLFs). Mesoporous NPs were synthesized in a basic environment via a surfactant assisted cooperative self-assembly process and were characterized using Scanning Electron Microscopy (SEM), X-ray Fluorescence Spectroscopy (XRF), Fourier Transform Infrared Spectroscopy (FT-IR), X-ray Diffraction Analysis (XRD) and N2 Porosimetry. The loading capacity of NPs was evaluated using Ultrahigh Performance Liquid Chromatography/High resolution Mass Spectrometry (UHPLC/HRMS). Their biocompatibility was evaluated with the MTT assay, and the analysis of reactive oxygen species was performed using the cell-permeable ROS-sensitive probe 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA). The synthesized NPs presented a mesoporous structure with a surface area ranging from 1312 m2/g for undoped silica to 495 m2/g for the Ce-doped NPs, excellent bioactivity after a 1-day immersion in c-SBF, hemocompatibility and a high loading capacity (around 80%). They presented ROS scavenging properties, and both the unloaded and ART-loaded NPs significantly promoted cell proliferation even at high concentrations of NPs (125 μg/mL). The ART-loaded Ce-doped NPs with the highest amount of cerium slightly restricted cell proliferation after 7 days of culture, but the difference was not significant compared with the control untreated cells. |
| Databáze: | OpenAIRE |
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