Rapid monitoring of plant growth regulators in bean sprouts via automated on-line polymeric monolith solid-phase extraction coupled with liquid chromatography tandem mass spectrometry
| Autor: | Lian-Feng Ai, Manman Wang, Lei Zhang, Dongmei Li, Xue-Lei Chen, Qixun Nian, Xuesheng Wang |
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| Rok vydání: | 2018 |
| Předmět: |
Time Factors
Monolithic HPLC column 010402 general chemistry 01 natural sciences Biochemistry Naphthaleneacetic Acids Analytical Chemistry Adsorption Plant Growth Regulators Limit of Detection Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Solid phase extraction Monolith Chromatography High Pressure Liquid Detection limit geography Chromatography geography.geographical_feature_category Chemistry Vigna Solid Phase Extraction 010401 analytical chemistry Extraction (chemistry) Equipment Design Gibberellins 0104 chemical sciences Linear range Seedlings Soybeans 2 4-Dichlorophenoxyacetic Acid |
| Zdroj: | Analytical and Bioanalytical Chemistry. 410:7239-7247 |
| ISSN: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-018-1334-x |
| Popis: | An automated on-line solid-phase extraction (SPE) following liquid chromatography tandem mass spectrometry was established for the fast determination of plant growth regulator residues in soybean sprout and mung bean sprout. The crude extracted specimens were directly purified on a poly (2-(dimethylamino) ethyl methacrylate-co-ethylene dimethacrylate) monolithic column which was well-defined as the on-line SPE adsorbent. Under the optimized conditions, the developed method gave the linear range of 0.3-50 ng/mL for gibberellin and 2,4-dichlorophenoxyacetic acid, 0.2-50 ng/mL for 4-chlorophenoxyacetic acid, and 0.5-50 ng/mL for 1-naphthaleneacetic acid (r ≥ 0.998). The detection limits (S/N = 3) ranged from 1.0 to 2.5 μg/kg and the recoveries for spiked soybean sprout samples were in the range of 75.0-93.3%. Besides, the total time for one analysis was 16 min. The reusability of the monolith was up to 600 extractions. The proposed process facilitated fully automated SPE and accurate determination in one step with rapidity, simplicity, and reliability. Graphical abstract ᅟ. |
| Databáze: | OpenAIRE |
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