Comprehensive Multiphase (CMP) NMR Monitoring of the Structural Changes and Molecular Flux Within a Growing Seed
| Autor: | A. Gorissen, Malcolm M. Campbell, Blythe Fortier-McGill, Heather L. Wheeler, Myrna J. Simpson, Ries de Visser, André J. Simpson, Yalda Liaghati-Mobarhan, Leayen Lam, Ronald Soong, Andre Sutrisno, Rudraksha Dutta Majumdar |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine In situ Magnetic Resonance Spectroscopy Germination Biology 01 natural sciences 03 medical and health sciences Metabolomics Phase (matter) Botany Triticum Chromatography food and beverages General Chemistry Nuclear magnetic resonance spectroscopy Carbon-13 NMR carbohydrates (lipids) 030104 developmental biology Seedlings Seeds General Agricultural and Biological Sciences Flux (metabolism) Heteronuclear single quantum coherence spectroscopy 010606 plant biology & botany |
| Zdroj: | Journal of Agricultural and Food Chemistry. 65:6779-6788 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.7b02421 |
| Popis: | A relatively recent technique termed comprehensive multiphase (CMP) NMR spectroscopy was used to investigate the growth and associated metabolomic changes of 13C-labeled wheat seeds and germinated seedlings. CMP-NMR enables the study of all phases in intact samples (i.e., liquid, gel-like, semisolid, and solid), by combining all required electronics into a single NMR probe, and can be used for investigating biological processes such as seed germination. All components, from the most liquid-like (i.e., dissolved metabolites) to the most rigid or solid-like (seed coat) were monitored in situ over 4 days. A wide range of metabolites were identified, and after 96 h of germination, the number of metabolites in the mobile phase more than doubled in comparison to 0 h (dry seed). This work represents the first application of CMP-NMR to follow biological processes in plants. |
| Databáze: | OpenAIRE |
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