Steroid Inhibitory Effects upon Human Adrenal 3βHydroxysteroid Dehydrogenase Activity*
| Autor: | G.C. Byrne, Y.S. Perry, J.S.D Winter |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Adult
medicine.medical_specialty 3-Hydroxysteroid Dehydrogenases Adolescent Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry Dehydroepiandrosterone In Vitro Techniques Biochemistry Substrate Specificity Steroid Endocrinology Microsomes Internal medicine Adrenal Glands medicine Humans Child Hydrocortisone biology Adrenal cortex Biochemistry (medical) Middle Aged Enzyme assay Kinetics Steroid hormone medicine.anatomical_structure biology.protein Microsome Pregnenolone Steroids medicine.drug |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 62:413-418 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jcem-62-2-413 |
| Popis: | The inhibitory effects of varying concentrations of steroids upon 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase (3 beta-HSD) kinetics were studied in human adrenal microsomes. Each enzyme assay was conducted in triplicate at five different concentrations of three substrates (dehydroepiandrosterone, pregnenolone, and 17OH-pregnenolone), using microsomes from at least three donors. Each steroid was screened for possible inhibition at concentrations of 10(-8) and 10(-6) M and then studied in more detail at five different concentrations. The type of inhibition and the inhibition constant (Ki) were determined by analysis of Lineweaver-Burk and Dixon plots, together with replots of the slopes from the Dixon plots. The mean Km (Michaelis-Menten constant) for the three substrates was 0.42 +/- 0.04 (SE) mumol/liter (n = 73). Each steroid tested, including delta 5-3 beta-hydroxysteroids, estrogens, and several delta 4-3-ketosteroids, with the exception of cortisol, caused significant inhibition of 3 beta-HSD activity, and in each case the steroid appeared to behave as a competitive inhibitor. In most cases the Ki value was approximately 10(-7) M. At micromolar concentrations several steroids, notably estrone and estradiol, caused almost total inhibition of adrenal 3 beta-HSD activity. Comparison of the calculated Ki values with available data concerning changes in intra-adrenal steroid concentrations during childhood suggests that these changes would be sufficient to cause a relative decline in 3 beta-HSD activity during adrenarche. Although postnatal circulating steroid concentrations would appear to be insufficient to influence adrenal steroidogenesis, the high serum levels of placental steroids during fetal life would be expected to cause marked 3 beta-HSD inhibition. |
| Databáze: | OpenAIRE |
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