Mechanistic effect of human umbilical cord blood derived mesenchymal stem cells on the submandibular salivary gland in ovariectomized rats
| Autor: | Samia Hussein, Zienab A. Gouda, Mohamed E. Ali Khalifa, Sally M. Shalaby |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Ovariectomy Biology Biochemistry Umbilical cord Salivary Glands 03 medical and health sciences stomatognathic system Internal medicine medicine Animals Humans Rats Wistar Molecular Biology Cells Cultured Salivary gland Mesenchymal stem cell Mesenchymal Stem Cells Cell Biology Fetal Blood Flow Cytometry Submandibular gland Rats 030104 developmental biology medicine.anatomical_structure Endocrinology Ovariectomized rat Female |
| Zdroj: | Biochemistry and Cell Biology. 96:57-67 |
| ISSN: | 1208-6002 0829-8211 |
| Popis: | We performed this study to understand the effect of human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) on the submandibular gland after bilateral ovariectomy. For this, 21 adult female rats were distributed equally among 3 groups: the sham-operated group (SHAM); the ovariectomized group (OVX); and the OVX group that received repeated intravenous injections of the hUCB-MSCs (OVX + hUCB-MSCs). We used reverse transcription – PCR to analyze for the gene expression of AQPs 3, 4, 5, and BMP-6. The cellular localization and expression of human CD105, human CD34, proliferating nuclear antigen (PCNA), single-stranded DNA (ss-DNA), caspase 3, AQP1, and α smooth muscle actin (α-SMA) were determined immunohistochemically. In the OVX group, a significant decrease in the gene expression of AQP3, AQP4, and BMP6, as well as the acinar area % was detected, while area % of granular convoluted tubules (GCTs) showed a significant increase. A significant decrease in area % staining positively for AQP1 and α-SMA was noted. An obvious improvement in the structure of the submandibular gland was demonstrated in the group injected with hUCB-MSCs, as well as a significant increase in the gene expression of AQP3, AQP4, and BMP6. The acinar and GCT area %, as well as the different measured markers, were relatively normal. This demonstrates that E2-deficiency induces structural changes to the submandibular gland. Moreover, a definite amelioration of the structure and function of the submandibular gland was detected after the administration of hUCB-MSCs. |
| Databáze: | OpenAIRE |
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