HFE gene variants and iron-induced oxygen radical generation in idiopathic pulmonary fibrosis
| Autor: | Federica Sangiuolo, Francesca Mariani, Ermanno Puxeddu, Alessia Comandini, Giovanni Simonetti, Marco Pallante, Monica Losi, Cesare Saltini, Gabriella Pezzuto, Francesco Cavalli, Gianluigi Sergiacomi, Augusto Orlandi, Donato Di Pierro, Massimo Amicosante, A. Bisetti, Andrea Magrini, Giuliana Longo, Maurizio Zompatori, Daniela Fraboni |
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| Přispěvatelé: | Sangiuolo F, Puxeddu E, Pezzuto G, Cavalli F, Longo G, Comandini A, Di Pierro D, Pallante M, Sergiacomi G, Simonetti G, Zompatori M, Orlandi A, Magrini A, Amicosante M, Mariani F, Losi M, Fraboni D, Bisetti A, Saltini C |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Pulmonary and Respiratory Medicine Pathology medicine.medical_specialty Iron Settore MED/10 - Malattie dell'Apparato Respiratorio Cell Hemosiderin Bronchoalveolar Lavage Idiopathic pulmonary fibrosis chemistry.chemical_compound Fibrosis Malondialdehyde medicine Extracellular Humans Fluorometry Hemochromatosis Protein Alleles Chromatography High Pressure Liquid Inflammation Settore MED/04 - Patologia Generale Lung medicine.diagnostic_test business.industry Macrophages Histocompatibility Antigens Class I Wild type Genetic Variation Membrane Proteins Middle Aged respiratory system medicine.disease Molecular biology Oxygen medicine.anatomical_structure Bronchoalveolar lavage chemistry Settore MED/03 - Genetica Medica IDIOPATHIC PULMONARY FIBROSIS Case-Control Studies UIP Female Hemochromatosis Reactive Oxygen Species business Bronchoalveolar Lavage Fluid |
| Popis: | In idiopathic pulmonary fibrosis (IPF), lung accumulation of excessive extracellular iron and macrophage haemosiderin may suggest disordered iron homeostasis leading to recurring microscopic injury and fibrosing damage.The current study population comprised 89 consistent IPF patients and 107 controls. 54 patients and 11 controls underwent bronchoalveolar lavage (BAL). Haemosiderin was assessed by Perls' stain, BAL fluid malondialdehyde (MDA) by high-performance liquid chromatography, BAL cell iron-dependent oxygen radical generation by fluorimetry and the frequency of hereditary haemochromatosis HFE gene variants by reverse dot blot hybridisation.Macrophage haemosiderin, BAL fluid MDA and BAL cell unstimulated iron-dependent oxygen radical generation were all significantly increased above controls (pversus22.4%, OR 2.35, p=0.008) and was associated with higher iron-dependent oxygen radical generation (HFE variant 107.4±56.0, HFE wild type (wt) 59.4±36.4 and controls 16.7±11.8 fluorescence units per 105BAL cells; p=0.028 HFE variantversusHFE wt, p=0.006 HFE wtversuscontrols).The data suggest iron dysregulation associated with HFE allelic variants may play an important role in increasing susceptibility to environmental exposures, leading to recurring injury and fibrosis in IPF. |
| Databáze: | OpenAIRE |
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