Transgenic expression of interleukin 7 restores T cell populations in nude mice
| Autor: | B E Rich, Philip Leder |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Receptor complex
CD8 Antigens T-Lymphocytes T cell Immunology Mice Nude Mice Transgenic Mice Nude mouse medicine Animals Immunology and Allergy Cytotoxic T cell Mice Inbred BALB C biology Interleukin-7 T-cell receptor Lymphokine Interleukin Articles biology.organism_classification Molecular biology Rats medicine.anatomical_structure CD4 Antigens CD8 |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| Popis: | The thymic lesion of the nude mouse causes a profound block in T cell development. The failure of most T cells to mature in nude mice is likely to reflect a requirement for signals elaborated in the normal thymus. Interleukin 7 (IL-7), a lymphokine that is normally expressed in the thymus and has been implicated in T cell maturation, might be central to this process. To test this possibility, we introduced a transgene directing lymphoid expression of IL-7 into nude mice and found that it substantially alleviates the block in T cell maturation caused by the thymic defect. IL-7 transgenic nude mice have increased numbers of peripheral cells expressing the T cell marker Thy-1, the T cell antigen receptor complex, and the co-receptors CD4 and CD8. The IL-7 transgene also restores T cell-specific proliferation and activation responses to the peripheral cells of transgene-rescued nude mice. Such findings point toward a fundamental role for IL-7 in the thymic maturation of T cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|