TonEBP recognizes R-loops and initiates m6A RNA methylation for R-loop resolution
| Autor: | Jun Ho Lee, Hyug Moo Kwon, Byeong Jin Ye, Hongtae Kim, Kyungjae Myung, Soo Youn Choi, Hyun Park, Jin-Hoe Hur, Gyu Won Jeong, Na Young Cheon, Ja Yil Lee, Eun Jin Yoo, Hyun Je Kang, Eun-A. Lee, KS Lee, Whaseon Lee-Kwon |
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| Rok vydání: | 2020 |
| Předmět: |
DNA Replication
Adenosine RNA methylation R-loop Ultraviolet Rays AcademicSubjects/SCI00010 Ribonuclease H Biology Genome Integrity Repair and Replication Methylation Rel homology domain Diffusion 03 medical and health sciences chemistry.chemical_compound DNA Adducts 0302 clinical medicine Enhancer binding Cell Line Tumor Protein Interaction Mapping Genetics Humans 030304 developmental biology 0303 health sciences RNA DNA Methyltransferases Cell biology HEK293 Cells chemistry Nucleic acid R-Loop Structures 030217 neurology & neurosurgery DNA Damage Protein Binding Transcription Factors |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 |
| Popis: | R-loops are three-stranded, RNA–DNA hybrid, nucleic acid structures produced due to inappropriate processing of newly transcribed RNA or transcription-replication collision (TRC). Although R-loops are important for many cellular processes, their accumulation causes genomic instability and malignant diseases, so these structures are tightly regulated. It was recently reported that R-loop accumulation is resolved by methyltransferase-like 3 (METTL3)-mediated m6A RNA methylation under physiological conditions. However, it remains unclear how R-loops in the genome are recognized and induce resolution signals. Here, we demonstrate that tonicity-responsive enhancer binding protein (TonEBP) recognizes R-loops generated by DNA damaging agents such as ultraviolet (UV) or camptothecin (CPT). Single-molecule imaging and biochemical assays reveal that TonEBP preferentially binds a R-loop via both 3D collision and 1D diffusion along DNA in vitro. In addition, we find that TonEBP recruits METTL3 to R-loops through the Rel homology domain (RHD) for m6A RNA methylation. We also show that TonEBP recruits RNaseH1 to R-loops through a METTL3 interaction. Consistent with this, TonEBP or METTL3 depletion increases R-loops and reduces cell survival in the presence of UV or CPT. Collectively, our results reveal an R-loop resolution pathway by TonEBP and m6A RNA methylation by METTL3 and provide new insights into R-loop resolution processes. |
| Databáze: | OpenAIRE |
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