Enzyme immunoassay for detection of antibodies against isolated flagella ofLegionella pneumophila
| Autor: | Joerg Hacker, B. Nowak, Reinhard Marre, B. Neumeister, G. Ruckdeschel, Milorad Susa, Eberhard Straube |
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| Rok vydání: | 1995 |
| Předmět: |
Microbiology (medical)
Legionella medicine.disease_cause Legionella pneumophila Microbiology Immunoenzyme Techniques Antigen Legionella gormanii medicine Humans Legionella pneumophila Serogroup 1 Fluorescent Antibody Technique Indirect biology medicine.diagnostic_test General Medicine bacterial infections and mycoses biology.organism_classification Antibodies Bacterial Virology respiratory tract diseases Titer Infectious Diseases Flagella Immunoassay biology.protein bacteria Antibody |
| Zdroj: | European Journal of Clinical Microbiology & Infectious Diseases. 14:764-767 |
| ISSN: | 1435-4373 0934-9723 |
| DOI: | 10.1007/bf01690990 |
| Popis: | Flagella of Legionella pneumophila serogroup 1, strain Philadelphia (ATCC 33152), were isolated and used as antigen to detect antibodies by enzyme immunoassay (EIA) in sera of patients with significant titers against Legionella as determined by the indirect immunofluorescence assay (IFA). Healthy blood donors served as a control group. Whereas the sera of blood donors were negative in both assays, sera of patients showed two patterns of reactivity. Sera with elevated titers against Legionella pneumophila serogroup 1 or Legionella gormanii in IFA usually demonstrated high titers in EIA. However, high IFA titers against Legionella bozemanii were associated with low titers in EIA. The data show that flagella protein of Legionella pneumophila serogroup 1 strain Philadelphia is of limited value for the detection of antibodies against Legionella other than Legionella pneumophila serogroup 1, although in vitro studies revealed genus-specific epitopes. This might be due to unpredictable variabilities in the expression of flagella in vivo. |
| Databáze: | OpenAIRE |
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