The age-related resistance of rats to Plasmodium berghei infection is associated with differential cellular and humoral immune responses
| Autor: | Estelle Adam, Jamal Khalife, C. Godin, Sophia Lafitte, Abdelhadi Saoudi, Monique Capron, Christine Pierrot |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Adoptive cell transfer Aging Plasmodium berghei Antibodies Protozoan Age-related resistance Parasitemia Interferon-gamma Immune system T-Lymphocyte Subsets Animals IL-2 receptor B-Lymphocytes biology Macrophages Natural killer T cell biology.organism_classification Adoptive Transfer Rats Inbred F344 Interleukin-10 Malaria Rats Killer Cells Natural Infectious Diseases Immunology biology.protein Cytokines Parasitology Disease Susceptibility Antibody CD8 Spleen |
| Zdroj: | International journal for parasitology. 33(10) |
| ISSN: | 0020-7519 |
| Popis: | In this study, we investigated how the age of rats would affect the course of infection of and the immune response to Plasmodium berghei . Both young (4-week-old) and adult rats (8-week-old) can be infected with P. berghei ANKA strain, with significantly higher levels of infected red blood cells in young rats. While 100% of young rats succumbed to infection, adult rats were able to clear blood parasites and no mortality was observed. Analysis of cellular distribution and circulating cytokines demonstrated the persistence of CD4+/CD25+ T cells and high expression of circulating interleukin-10 (IL-10) during the progression of infection in young-susceptible rats, whereas high levels of CD8+ T cells and natural killer T cells are detected in adult-resistant rats. Analysis of antibody isotypes showed that adult rats produced significantly higher levels of interferon-γ (IFN-γ)-dependent IgG2c antibodies than young rats during infection. Further evaluation of the role of IL-10, IFN-γ and of immune cells showed that only the adoptive transfer of spleen cells from adult-resistant rats was able to convert susceptibility of young-susceptible rats to a resistant phenotype. These observations suggest that cell-mediated mechanisms are crucial for the control of a primary infection with P. berghei in young rats. |
| Databáze: | OpenAIRE |
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