Low-dose oral ferrous fumarate aggravated intestinal inflammation in rats with DSS-induced colitis
| Autor: | Trygve Hausken, Anne Marita Milde, Kari Erichsen, Oddrun Anita Gudbrandsen, Lars Helgeland, Gülen Arslan, Arnold Berstad, Rune J. Ulvik, Rolf K. Berge |
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| Rok vydání: | 2005 |
| Předmět: |
Vitamin
Male Administration Oral Pharmacology medicine.disease_cause Sensitivity and Specificity Ferrous Fumarate Ferrous chemistry.chemical_compound Random Allocation Intestinal mucosa Reference Values medicine Confidence Intervals Immunology and Allergy Animals Ferrous Compounds Colitis Intestinal Mucosa Rats Wistar Probability Analysis of Variance Dose-Response Relationship Drug Biopsy Needle Dextran Sulfate Gastroenterology medicine.disease Malondialdehyde Immunohistochemistry Rats stomatognathic diseases Disease Models Animal Oxidative Stress chemistry Immunology Colitis Ulcerative Oxidative stress medicine.drug |
| Zdroj: | Inflammatory bowel diseases. 11(8) |
| ISSN: | 1078-0998 |
| Popis: | Background: Oral ferrous iron therapy may reinforce intestinal inflammation. One possible mechanism is by catalyzing the production of reactive oxygen species. We studied the effects of low-dose oral ferrous fumarate on intestinal inflammation and plasma redox status in dextran sulfate sodium (DSS)-induced colitis in rats. Methods: Forty male Wistar rats were divided into 5 groups: no intervention, sham gavage (distilled water), ferrous fumarate, DSS, and ferrous fumarate + DSS. Ferrous fumarate was dissolved in distilled water (0.60 mg Fe 2+ /kg per day) and administered by gavage on days 1 to 14. All rats were fed a standard diet. Colitis was induced by 5% DSS in drinking water on days 8 to 14. Rats were killed on day 16. Histologic colitis scores, fecal granulocyte marker protein, plasma malondialdehyde, plasma antioxidant vitamins, and plasma aminothiols were measured. Results: DSS significantly increased histologic colitis scores (P , 0.001) and fecal granulocyte marker protein (P , 0.01). Ferrous fumarate further increased histologic colitis scores (P , 0.01) in DSS-induced colitis. DSS + ferrous fumarate decreased plasma vitamin A compared with controls (P , 0.01). Otherwise, no changes were seen in plasma malondialdehyde, plasma antioxidant vitamins, or plasma aminothiols. Conclusion: Low-dose oral ferrous iron enhanced intestinal inflammation in DSS-induced colitis in rats. |
| Databáze: | OpenAIRE |
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