Cloning of the PYR4 gene encoding orotidine-5?-phosphate decarboxylase in Cephalosporium acremonium
| Autor: | Miguel A. Peñalva, Alejandro Vian |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Carboxy-Lyases
Genes Fungal Molecular Sequence Data Orotidine-5'-Phosphate Decarboxylase Restriction Mapping Mutant Homology (biology) Neurospora crassa Conserved sequence Gene product Sequence Homology Nucleic Acid Genetics Amino Acid Sequence Cloning Molecular Gene Orotidine 5'-phosphate decarboxylase biology Acremonium Nucleic Acid Hybridization General Medicine biology.organism_classification Biological Evolution Biochemistry Electrophoresis Polyacrylamide Gel |
| Zdroj: | Current Genetics. 17:223-227 |
| ISSN: | 1432-0983 0172-8083 |
| DOI: | 10.1007/bf00312613 |
| Popis: | We have cloned the Cephalosporium acremonium pyr4 gene by cross-hybridization with the equivalent gene from Neurospora crassa, the closest relative from which this gene is available. The C. acremonium pyr4 gene complements an E. coli pyrF mutant lacking orotidine-5'-phosphate decarboxylase (OMPdecase), and most probably does not contain introns. Maxicell analysis in E. coli shows that it encodes a 46 kDa polypeptide. The C. acremonium OMPdecase contains a highly conserved pentadecapeptide characteristic for this category of enzyme. Extensive sequence comparison suggests an important role of this region in enzymatic activity. |
| Databáze: | OpenAIRE |
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