Increased vimentin in human α- and β-cells in type 2 diabetes
| Autor: | Anne Clark, Alexander Hamilton, Melissa F. Brereton, Michael Verschoor, Joanna M Williams Durkin, Laura J. McCulloch, Eelco J.P. de Koning, F. Carlotti, M. Engelse, Hannah J. Wills, Kevin Docherty, Maaike M Roefs, Laura Garcia-Perez, Barbara C. Hansen, Katherine Elizabeth Jones, Paul Johnson |
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| Přispěvatelé: | Hubrecht Institute for Developmental Biology and Stem Cell Research |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Cells Endocrinology Diabetes and Metabolism medicine.medical_treatment Vimentin Type 2 diabetes 03 medical and health sciences Endocrinology Hyperinsulinism Insulin-Secreting Cells Internal medicine Diabetes mellitus Diabetes Mellitus Journal Article Animals Humans Medicine Cells Cultured geography Cultured geography.geographical_feature_category biology business.industry Insulin Islet medicine.disease Macaca mulatta Macaca fascicularis 030104 developmental biology medicine.anatomical_structure Diabetes Mellitus Type 2 Glucagon-Secreting Cells Case-Control Studies biology.protein PDX1 business Pancreas Type 2 |
| Zdroj: | The Journal of endocrinology, 233(3), 217-227. Society for Endocrinology |
| ISSN: | 1479-6805 0022-0795 |
| DOI: | 10.1530/joe-16-0588 |
| Popis: | Type 2 diabetes (T2DM) is associated with pancreatic islet dysfunction. Loss of β-cell identity has been implicated via dedifferentiation or conversion to other pancreatic endocrine cell types. How these transitions contribute to the onset and progression of T2DM in vivo is unknown. The aims of this study were to determine the degree of epithelial-to-mesenchymal transition occurring in α and β cells in vivo and to relate this to diabetes-associated (patho)physiological conditions. The proportion of islet cells expressing the mesenchymal marker vimentin was determined by immunohistochemistry and quantitative morphometry in specimens of pancreas from human donors with T2DM (n = 28) and without diabetes (ND, n = 38) and in non-human primates at different stages of the diabetic syndrome: normoglycaemic (ND, n = 4), obese, hyperinsulinaemic (HI, n = 4) and hyperglycaemic (DM, n = 8). Vimentin co-localised more frequently with glucagon (α-cells) than with insulin (β-cells) in the human ND group (1.43% total α-cells, 0.98% total β-cells, median; P P P |
| Databáze: | OpenAIRE |
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