The extraction of polymeric collagen from biopsies of human skin
| Autor: | M.J.O. Francis, D.C. MacMillan |
|---|---|
| Rok vydání: | 1971 |
| Předmět: |
Adult
Protein Denaturation Chemistry Skin specimen Biopsy Extraction (chemistry) Human skin Anatomy Middle Aged Biochemistry Genetics and Molecular Biology (miscellaneous) Pronase Lyotropic Ultrastructure Relaxation (physics) Humans Indicators and Reagents Collagen alpha-Amylases Biomedical engineering Aged Skin |
| Zdroj: | Biochimica et biophysica acta. 251(2) |
| ISSN: | 0006-3002 |
| Popis: | 1. 1. Medium sized biopsies (100 mm2) of human skin from 14 subjects yielded sufficient polymeric collagen for depolymerisation and ultrastructural investigations. 2. 2. The yields obtained from one skin specimen by the α-amylase, EDTA and lyotropic relaxation (water) methods of extracting polymeric collagen are similar. 3. 3. The responses to depolymerisation treatments of the three polymeric collagen samples extracted by each of the three methods from one skin specimen are cross-correlated. There are however electron microscopical differences between the three polymeric collagen samples. 4. 4. The results show that it feasible to study the polymeric collagen of normal and diseased human skin from medium sized biopsies. |
| Databáze: | OpenAIRE |
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