Graft revascularization is essential for non-invasive monitoring of transplanted islets with radiolabeled exendin
| Autor: | Wael A. Eter, Martin Gotthardt, Desiree Bos, Maarten Brom, Otto C. Boerman, Cathelijne Frielink |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Pathology endocrine system endocrine system diseases medicine.medical_treatment Islets of Langerhans Transplantation 030209 endocrinology & metabolism Revascularization Article Diabetes Mellitus Experimental 03 medical and health sciences Mice 0302 clinical medicine In vivo Spect imaging Tumours of the digestive tract Radboud Institute for Molecular Life Sciences [Radboudumc 14] medicine Animals Humans 030304 developmental biology Tomography Emission-Computed Single-Photon 0303 health sciences Type 1 diabetes geography Multidisciplinary geography.geographical_feature_category business.industry Other Research Radboud Institute for Health Sciences [Radboudumc 0] Non invasive Graft Survival Indium Radioisotopes medicine.disease Islet 3. Good health Surgery Transplantation Radiography surgical procedures operative Diabetes Mellitus Type 1 business Peptides Nanomedicine Radboud Institute for Molecular Life Sciences [Radboudumc 19] |
| Zdroj: | Scientific Reports Scientific Reports, 5 |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep15521 |
| Popis: | Islet transplantation is a novel promising strategy to cure type 1 diabetes. However, the long-term outcome is still poor, because both function and survival of the transplant decline over-time. Non-invasive imaging methods have the potential to enable monitoring of islet survival after transplantation and the effects of immunosuppressive drugs on transplantation outcome. 111In-labeled exendin-3 is a promising tracer to visualize native and transplanted islets by SPECT (Single Photon Emission Computed Tomography). In the present study, we hypothesized that islet microvasculature plays an important role determining the uptake of exendin-3 in islets when monitoring transplant survival. We observed 111In-exendin-3 accumulation in the transplant as early as three days after transplantation and an increase in the uptake up to three weeks post-transplantation. Islet-revascularization correlated with the increase in 111In-exendin-3 uptake, whereas fully re-established islet vasculature coincided with a stabilized uptake of the radiotracer in the transplant. Here, we demonstrate the importance of islet vasculature for in vivo delivery of radiotracers to transplanted islets and we demonstrate that optimal and stable uptake of exendin four weeks after transplantation opens the possibility for long-term monitoring of islet survival by SPECT imaging. |
| Databáze: | OpenAIRE |
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